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Literature summary for 2.4.2.38 extracted from
- Expression of eukaryotic glycosyltransferases in the yeast Pichia pastoris (2003), Biochimie, 85, 413-422.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of N-terminally His5-tagged enzyme in Pichia pastoris, recombinant enzyme is secreted to the medium | Arabidopsis thaliana |
General Stability
| General Stability | Organism |
|---|---|
| the purified recombinant enzyme is not stable to a single freeze-thaw cycle | Arabidopsis thaliana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Arabidopsis thaliana |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His5-tagged enzyme from Pichia pastoris cell medium by nickel chelating affinity chromatography and ultrafiltration to homogeneity | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.33 | - |
purified recombinant enzyme | Arabidopsis thaliana |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified recombinant enzyme, 3 weeks stable without loss of activity | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-xylose + dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc | i.e. dansylated GnGn-peptide | Arabidopsis thaliana | UDP + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta1,2-xylosyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 16 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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