Literature summary for 2.4.2.37 extracted from

Huergo, L.F.; Merrick, M.; Monteiro, R.A.; Chubatsu, L.S.; Steffens, M.B.; Pedrosa, F.O.; Souza, E.M.
In vitro interactions between the PII proteins and the nitrogenase regulatory enzymes dinitrogenase reductase ADP-ribosyltransferase (DraT) and dinitrogenase reductase-activating glycohydrolase (DraG) in Azospirillum brasilense (2009), J. Biol. Chem., 284, 6674-6682.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	intracellular fluctuation of the PII ligands play a key role in the post-translational regulation of activity	Azospirillum brasilense
protein Gln B	regulates DraT activity. GlnB promotes a significant solubilization of HisDraT, which again suggests a possible conformational change in DraT upon GlnB binding. HisDraT has lower affinity for Ni2+ when complexed with GlnB. DraT-GlnB complex is more stable in ADP	Azospirillum brasilense

Cloned(Commentary)

Cloned (Comment)	Organism
plasmids pLHPETDraT and pLHDK5pII co-expressed in Escherichia coli BL21	Azospirillum brasilense

Inhibitors

Inhibitors	Comment	Organism	Structure
2-oxoglutarate	destabilizes the DraT-GlnB complex	Azospirillum brasilense
ATP	destabilizes the DraT-GlnB complex	Azospirillum brasilense
additional information	intracellular fluctuation of the PII ligands play a key role in the post-translational regulation of activity	Azospirillum brasilense

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	x * 37000, SDS-PAGE	Azospirillum brasilense

Organism

Organism	UniProt	Comment	Textmining
Azospirillum brasilense	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisDraT-GlnB complex formed in vivo purified to homogeneity in the presence of ADP, on Ni2+ column	Azospirillum brasilense

Storage Stability

Storage Stability	Organism
-80°C	Azospirillum brasilense

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Azospirillum brasilense

Synonyms

Synonyms	Comment	Organism
dinitrogenase reductase ADP-ribosyltransferase	-	Azospirillum brasilense
DRAT	-	Azospirillum brasilense

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Release 2023.1 (January 2023)