BRENDA - Enzyme Database show
show all sequences of 2.4.2.37

Purification and properties of dinitrogenase reductase ADP-ribosyltransferase from the photosynthetic bacterium Rhodospirillum rubrum

Lowery, R.G.; Ludden, P.W.; J. Biol. Chem. 263, 16714-16719 (1988)

Data extracted from this reference:

General Stability
General Stability
Organism
ADP and NaCl stabilize during purification and storage
Rhodospirillum rubrum
desalting procedures during purification inactivate
Rhodospirillum rubrum
DTT stabilizes during purification
Rhodospirillum rubrum
extremely unstable in crude extract or partially purified preparation
Rhodospirillum rubrum
one freeze-thawing cycle leads to 50% loss of activity, ADP stabilizes
Rhodospirillum rubrum
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-aminobenzamide
-
Rhodospirillum rubrum
KCl
reversible
Rhodospirillum rubrum
NaBr
strong
Rhodospirillum rubrum
NaCl
reversible
Rhodospirillum rubrum
NADH
-
Rhodospirillum rubrum
NADP+
-
Rhodospirillum rubrum
nicotinamide
-
Rhodospirillum rubrum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
NAD+
-
Rhodospirillum rubrum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Rhodospirillum rubrum
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
1 * 29000, SDS-PAGE
Rhodospirillum rubrum
30900
-
gel filtration
Rhodospirillum rubrum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + [dinitrogen reductase]
Rhodospirillum rubrum
involved in regulation of nitrogenase EC 1.18.6.1 activity
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodospirillum rubrum
-
-
-
Purification (Commentary)
Commentary
Organism
to near homogeneity
Rhodospirillum rubrum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.077
-
-
Rhodospirillum rubrum
Storage Stability
Storage Stability
Organism
-80°C, in liquid N2
Rhodospirillum rubrum
0°C, in 0.2 M NaCl and 1 mM ADP, 18 h
Rhodospirillum rubrum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
etheno-NAD+ + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
NAD+ + [dinitrogen reductase]
high specificity for acceptor substrate, the only acceptor is dinitrogen reductase
489801
Rhodospirillum rubrum
nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
NAD+ + [dinitrogen reductase]
involved in regulation of nitrogenase EC 1.18.6.1 activity
489801
Rhodospirillum rubrum
?
-
-
-
?
nicotinamide guanine dinucleotide + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
nicotinamide hypoxanthine dinucleotide + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE
Rhodospirillum rubrum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rhodospirillum rubrum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Rhodospirillum rubrum
General Stability (protein specific)
General Stability
Organism
ADP and NaCl stabilize during purification and storage
Rhodospirillum rubrum
desalting procedures during purification inactivate
Rhodospirillum rubrum
DTT stabilizes during purification
Rhodospirillum rubrum
extremely unstable in crude extract or partially purified preparation
Rhodospirillum rubrum
one freeze-thawing cycle leads to 50% loss of activity, ADP stabilizes
Rhodospirillum rubrum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-aminobenzamide
-
Rhodospirillum rubrum
KCl
reversible
Rhodospirillum rubrum
NaBr
strong
Rhodospirillum rubrum
NaCl
reversible
Rhodospirillum rubrum
NADH
-
Rhodospirillum rubrum
NADP+
-
Rhodospirillum rubrum
nicotinamide
-
Rhodospirillum rubrum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
NAD+
-
Rhodospirillum rubrum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Rhodospirillum rubrum
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
1 * 29000, SDS-PAGE
Rhodospirillum rubrum
30900
-
gel filtration
Rhodospirillum rubrum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + [dinitrogen reductase]
Rhodospirillum rubrum
involved in regulation of nitrogenase EC 1.18.6.1 activity
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
to near homogeneity
Rhodospirillum rubrum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.077
-
-
Rhodospirillum rubrum
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, in liquid N2
Rhodospirillum rubrum
0°C, in 0.2 M NaCl and 1 mM ADP, 18 h
Rhodospirillum rubrum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
etheno-NAD+ + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
NAD+ + [dinitrogen reductase]
high specificity for acceptor substrate, the only acceptor is dinitrogen reductase
489801
Rhodospirillum rubrum
nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
NAD+ + [dinitrogen reductase]
involved in regulation of nitrogenase EC 1.18.6.1 activity
489801
Rhodospirillum rubrum
?
-
-
-
?
nicotinamide guanine dinucleotide + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
nicotinamide hypoxanthine dinucleotide + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE
Rhodospirillum rubrum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rhodospirillum rubrum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Rhodospirillum rubrum
Other publictions for EC 2.4.2.37
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722556
Moure
The nitrogenase regulatory enz ...
Azospirillum brasilense
J. Bacteriol.
195
279-286
2013
2
-
-
-
-
-
1
1
-
1
-
-
-
6
-
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
1
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2
2
731792
Nordlund
ADP-ribosylation, a mechanism ...
Rhodospirillum rubrum
FEBS J.
280
3484-3490
2013
-
-
-
-
-
-
1
-
-
-
1
1
-
1
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-
-
-
-
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1
1
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-
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1
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1
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1
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1
1
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1
1
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-
-
-
-
-
-
-
-
-
-
-
721351
Heiniger
How posttranslational modifica ...
Rhodopseudomonas palustris
Appl. Environ. Microbiol.
78
1023-1032
2012
-
-
-
-
-
-
-
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4
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2
2
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704624
Huergo
In vitro interactions between ...
Azospirillum brasilense
J. Biol. Chem.
284
6674-6682
2009
2
-
1
-
-
-
3
-
-
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1
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-
2
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1
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1
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1
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2
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1
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3
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1
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1
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1
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1
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673667
Huergo
Interactions between PII prote ...
Azospirillum brasilense
FEBS Lett.
580
5232-5236
2006
-
-
1
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-
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1
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2
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1
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1
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1
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1
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2
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660835
Huergo
Effects of over-expression of ...
Azospirillum brasilense
Arch. Microbiol.
183
209-217
2005
-
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1
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1
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3
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1
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1
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2
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1
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1
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1
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1
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2
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661730
Kim
Characterization of altered re ...
Rhodospirillum rubrum
FEBS Lett.
559
84-88
2004
-
-
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-
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1
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2
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1
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1
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1
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2
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489812
Zhang
Effect of PII and its homolog ...
Rhodospirillum rubrum
J. Bacteriol.
183
1610-1620
2001
-
-
-
-
-
-
-
-
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1
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1
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489813
Ma
Role of the dinitrogenase redu ...
Rhodospirillum rubrum
J. Bacteriol.
183
250-256
2001
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3
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489816
Zhang
Isolation and characterization ...
Rhodospirillum rubrum
Microbiology
147
193-202
2001
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1
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2
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489810
Halbleib
Regulation of dinitrogenase re ...
Rhodospirillum rubrum
J. Biol. Chem.
275
3493-3500
2000
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1
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3
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489815
Grunwald
ADP-ribosylation of variants o ...
Rhodospirillum rubrum
J. Bacteriol.
182
2597-2603
2000
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4
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489814
Kim
Correlation of activity regula ...
Rhodospirillum rubrum
J. Bacteriol.
181
1698-1702
1999
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1
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2
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489811
Grunwald
NAD-dependent crosslinking of ...
Rhodospirillum rubrum
J. Bacteriol.
179
3277-3283
1997
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1
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1
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489808
Grunwald
Posttranslational regulation o ...
Rhodospirillum rubrum
J. Bacteriol.
177
628-635
1995
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489809
Zhang
Comparison studies of dinitrog ...
Azospirillum brasilense, Rhodospirillum rubrum
J. Bacteriol.
177
2354-2359
1995
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489807
Zhang
Cloning, sequencing, mutagenes ...
Azospirillum brasilense, Rhodospirillum rubrum
J. Bacteriol.
174
3364-3369
1992
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2
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6
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489805
Fu
Cloning and expression of draT ...
Azospirillum lipoferum
Gene
86
95-98
1990
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1
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6
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1
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1
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489802
Fitzmaurice
Genes coding for the reversibl ...
Rhodospirillum rubrum, Rhodospirillum rubrum UR2
Mol. Gen. Genet.
218
340-347
1989
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1
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4
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4
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6
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1
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4
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6
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489803
Lowery
Effect of nucleotides on the a ...
Rhodospirillum rubrum
Biochemistry
28
4956-4961
1989
3
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1
2
3
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4
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1
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1
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1
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1
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3
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1
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1
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2
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3
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1
-
1
-
1
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489804
Fu
Functional expression of a Rho ...
Rhodospirillum rubrum
Gene
85
153-160
1989
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1
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5
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1
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1
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1
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489806
Fu
Posttranslational regulatory s ...
Azospirillum brasilense, Azospirillum brasilense Sp7, Rhodospirillum rubrum
J. Bacteriol.
171
4679-4685
1989
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1
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9
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3
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2
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1
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1
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1
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3
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2
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489801
Lowery
Purification and properties of ...
Rhodospirillum rubrum
J. Biol. Chem.
263
16714-16719
1988
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5
7
1
1
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2
1
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6
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1
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1
2
5
1
1
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1
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5
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7
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1
1
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2
1
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1
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1
2
5
1
1
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1
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