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Literature summary for 2.4.2.36 extracted from

  • Masuyer, G.
    Crystal structure of exotoxin A from Aeromonas pathogenic species (2020), Toxins, 12, 397 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.36

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli K-12 cells Aeromonas hydrophila

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 12% (w/v) polyethylene glycol 6000, 0.1 M MES pH 5.5, 0.1 M ammonium acetate Aeromonas hydrophila

Protein Variants

Protein Variants Comment Organism
E571A catalytically inactive Aeromonas hydrophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + diphthamide-[translation elongation factor 2] Aeromonas hydrophila
-
 nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S200 gel filtration Aeromonas hydrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + diphthamide-[translation elongation factor 2]
-
 Aeromonas hydrophila nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Aeromonas hydrophila

Synonyms

Synonyms Comment Organism
diphthamide-specific mono-ADP-ribosylating toxin
-
 Aeromonas hydrophila
exotoxin A
-
 Aeromonas hydrophila

General Information

General Information Comment Organism
physiological function the enzyme is an important virulence factor from Aeromonas pathogenic species Aeromonas hydrophila