Crystallization (Comment) | Organism |
---|---|
a 1.8 A crystal structure of cholix in complex with its natural substrate, nicotinamide adenine dinucleotide | Vibrio cholerae serotype O1 |
Protein Variants | Comment | Organism |
---|---|---|
DELTA424-634 | catalytic domain of cholix has much greater affinity for NAD+ than the full-length enzyme. 15fold higher ADP-ribosyltransferase activity than full-length enzyme | Vibrio cholerae serotype O1 |
E574A | mutation of catalytic fragment DELTA424-634: mutant does not have a large impact on NAD+-binding. Catalytic activity is 3fold reduced compared to catalytic fragment DELTA424-634 | Vibrio cholerae serotype O1 |
E574A/E581A | mutation of catalytic fragment DELTA424-634: mutant has low affinity for NAD+. Mutant shows no catalytic activity | Vibrio cholerae serotype O1 |
E579R | mutation of catalytic fragment DELTA424-634: NAD+ binding not changed significantly. Catalytic activity is 1.6fold reduced compared to catalytic fragment DELTA424-634 | Vibrio cholerae serotype O1 |
E581A | mutation of catalytic fragment DELTA424-634: mutant has low affinity for NAD+. Mutant shows no catalytic activity | Vibrio cholerae serotype O1 |
Y493A | mutation of catalytic fragment DELTA424-634: mutation of catalytic fragment DELTA424-634: mutant has little effect on NAD+-binding. Mutant shows a marked reduction in activity compared to catalytic fragment DELTA424-634 | Vibrio cholerae serotype O1 |
Y504A | mutation of catalytic fragment DELTA424-634: mutant has little effect on NAD+-binding. Mutant shows a marked reduction in activity compared to catalytic fragment DELTA424-634 | Vibrio cholerae serotype O1 |
Y504F | mutation of catalytic fragment DELTA424-634: mutant has little effect on NAD+-binding. Mutant has lower affinity for NAD+ than Y504A mutant. Mutant shows a mild reduction in activity compared to catalytic fragment DELTA424-634 | Vibrio cholerae serotype O1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-amino-1,8-naphthalimide | - |
Vibrio cholerae serotype O1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + elongation factor 2 | - |
Vibrio cholerae serotype O1 | nicotinamide + ADPribose-elongation factor 2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cholix | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Vibrio cholerae serotype O1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Vibrio cholerae serotype O1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.042 | - |
pH 7.9, 25°C | Vibrio cholerae serotype O1 | 4-amino-1,8-naphthalimide |