Literature summary for 2.4.2.36 extracted from

Jorgensen, R.; Wang, Y.; Visschedyk, D.; Merrill, A.R.
The nature and character of the transition state for the ADP-ribosyltransferase reaction (2008), EMBO Rep., 9, 802-809.

Application

Application	Comment	Organism
drug development	more potent therapeutics for treatment of bacterial diseases and infections through understanding of ADPRT reaction meachanism	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with NAD+ and eEF2, PDB: 3B8H (mutant E546A), 3B82 (mutant E546H), 3B78 (mutant R551H), 2ZIT (wild-type), specific interactions of active-site loop1 with NAD+ and diphthamide results in solvent cover for dinucleotide-binding pocket and coordinates and stabilizes NAD+ in the active-site cleft during ADPRT reaction (transition-state model), crystals are of space group P(1)2(1), C2 symmetry, unit cell parameters: a: 326.9-329.4, b: 68.1-69.2, c: 190.0-191.6, beta: 102.9-103.3°, precipitant: PEG-8K or PEG-10K, 6-8%, 1.25 mM NAD+ (in cryo-protection buffer, pH 6.0) soaked into crystals	Pseudomonas aeruginosa

Crystallization (Comment)

Organism

in complex with NAD+ and eEF2, PDB: 3B8H (mutant E546A), 3B82 (mutant E546H), 3B78 (mutant R551H), 2ZIT (wild-type), specific interactions of active-site loop1 with NAD+ and diphthamide results in solvent cover for dinucleotide-binding pocket and coordinates and stabilizes NAD+ in the active-site cleft during ADPRT reaction (transition-state model), crystals are of space group P(1)2(1), C2 symmetry, unit cell parameters: a: 326.9-329.4, b: 68.1-69.2, c: 190.0-191.6, beta: 102.9-103.3°, precipitant: PEG-8K or PEG-10K, 6-8%, 1.25 mM NAD+ (in cryo-protection buffer, pH 6.0) soaked into crystals

Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
D461A	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
D463A	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
E546A	reduced ADPRT activity compared to wild-type possibly due to leakage of aqueous solvent into binding pocket which prevents ADP-ribose transfer, no impaired NAD+ binding and glycohydrolase activity, Glu546 crucial for ADPRT activity of ExoA but not strictly conserved among toxin family members, part of active-site loop 3 (546-551), upon NAD+-binding Glu546 and Tyr481 (both connected by hydrogen bonds resulting in water molecule exclusion) in hydrogen bonding distance to nucleophilic N3 of diphthamide imidazole possibly increasing its nucleophilic character	Pseudomonas aeruginosa
E546A/R551A	double alanine mutant, almost complete loss of ADPRT activity not restored by mutations E546D/R551K or E546R/R551E	Pseudomonas aeruginosa
E546D	lesser ADPRT activity than E546A	Pseudomonas aeruginosa
E546F	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
E546H	see E546A	Pseudomonas aeruginosa
E546N	partial rescue of ADPRT activity compared to E546A	Pseudomonas aeruginosa
E546Q	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
E547A	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
E548A	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
E553A	impaired NAD+ binding and glycohydrolase activity, Glu553 is a crucial catalytic residue and conserved among toxin family members	Pseudomonas aeruginosa
G453A	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
G454A	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
G454A	reduced ADPRT activity compared to wild-type, Gly454 is part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
G549A	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
G550A	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
L462A	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
L552A	QuickChange mutagenesis	Pseudomonas aeruginosa
Q460A	reduced ADPRT activity compared to wild-type, Gln460 is part of active-site loop 1 (453-463), active-site loop1 flips towards diphthamide of eEF2 by a hinged action of Ala457 and Ala464 upon NAD+ binding which places Gln460 close to adenine phosphate of NAD+, interaction of Gln460 with A-phosphate of NAD+ possibly prevents hydrolysis of NAD+ to AMP or ADP	Pseudomonas aeruginosa
R456A	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
R458A	56-fold reduction in ADPRT activity, 53-fold reduction in GH activity, and 31-fold increased KD for NAD+ compared to wild-type, ADPRT activity sensitivity towards substitution in order Gln>Lys>Trp>Ala>His, Arg458 is part of active-site loop 1 (453-463) and implicated in NAD+ substrate docking and orientation, active-site loop1 flips towards diphthamide of eEF2 by a hinged action of Ala457 and Ala464 upon NAD+ binding which enables van der Waals interactions between Arg458 and the adenine base of NAD+	Pseudomonas aeruginosa
R458H	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
R458K	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
R458Q	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
R458W	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
R551A	ADPRT activity sensitive to replacements in order Ala>Lys>Gln>Glu>His>Cys, Arg551 is part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
R551C	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
R551E	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
R551H	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
R551K	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
R551Q	part of active-site loop 3 (546-551)	Pseudomonas aeruginosa
S459A	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa
V455A	part of active-site loop 1 (453-463)	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	NAD+	double mutant E546A/R551A, relative KD = 1.11 +/-0.03	Pseudomonas aeruginosa
additional information	-	NAD+	double mutant E546D/R551K, relative KD = 2.71 +/-0.74	Pseudomonas aeruginosa
additional information	-	NAD+	double mutant E546R/R551E, relative KD = 1.29 +/-0.49	Pseudomonas aeruginosa
additional information	-	NAD+	mutant D461A, KD = 1.03 +/-0.02 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant D463A, KD = 1.18 +/-0.04 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546A, relative KD = 1.77 +/-0.34	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546D, relative KD = 3.83 +/-0.89	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546F, relative KD = 1.97 +/-0.11	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546H, relative KD = 1.03 +/-0.09	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546N, relative KD = 2.14 +/-0.26	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546Q, relative KD = 2.31 +/-0.71	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E547A, relative KD = 1.97 +/-0.77	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E548A, relative KD = 2.69 +/-0.11	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E553A, relative KD = 2.57 +/-0.86	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G453A, KD = 4.85 +/-1.07 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G454A, KD = 6.25 +/-0.22 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G549A, relative KD = 8.00 +/-0.94	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G550A, relative KD = 6.60 +/-0.54	Pseudomonas aeruginosa
additional information	-	NAD+	mutant L462A, KD = 1.86 +/-0.10 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant L552A, relative KD = 4.43 +/-0.57	Pseudomonas aeruginosa
additional information	-	NAD+	mutant Q460A, KD = 4.30 +/-0.43 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R456A, KD = 3.56 +/-0.53 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458A, KD = 5.78 +/-0.18 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458H, KD = 31.02 +/-7.18 relative to wild-type (d)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458K, KD = 3.18 +/-0.23 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458Q, KD = 0.45 +/-0.06 relative to wild-type (e)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458W, KD = 11.26 +/-1.16 relative to wild-type (d)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551A, relative KD = 2.69 +/-0.03	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551C, relative KD = 5.57 +/-0.91	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551E, relative KD = 5.03 +/-0.51	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551H, relative KD = 2.29 +/-0.46	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551K, relative KD = 1.29 +/-0.03	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551Q, relative KD = 2.63 +/-0.39	Pseudomonas aeruginosa
additional information	-	NAD+	mutant S459A, KD = 1.59 +/-0.16 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant V455A, KD = 3.31 +/-0.24 relative to wild-type (c)	Pseudomonas aeruginosa
additional information	-	NAD+	wild-type, KD = 35 +/-3 microM, relative KD = 1.00 +/-0.09, estimated according to quenched intrinsic tryptophan fluorescence upon NAD+ binding to the active site	Pseudomonas aeruginosa
additional information	-	NAD+	wild-type, KD = 36 +/-8 microM (c), KD = 62 +/-2 microM (d), KD = 74 +/-16 microM, relative KD = 1.00 +/-0.03	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Pseudomonas aeruginosa	NAD+-dependent ADP-ribosyltransfer (ADPRT) including NAD+-glycohydrolysis (GH) activity, deadly virulent due to covalent modification and thus inactivation of proteins that are essential for the host	?	-	?
NAD+ + essential ribosomal elongation factor 2	Pseudomonas aeruginosa	transfer of ADP-ribose moiety (oxocarbenium ion) of NAD+ onto N3 of diphthamide imidazole of essential ribosomal elongation factor 2 (eEF2)	nicotinamide + H+ + ADP-ribosylated essential ribosomal elongation factor 2	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	P11439	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	NAD+-dependent ADP-ribosyltransfer (ADPRT) including NAD+-glycohydrolysis (GH) activity, deadly virulent due to covalent modification and thus inactivation of proteins that are essential for the host	Pseudomonas aeruginosa	?	-	?
additional information	no ADP-ribosyl transfer by mutants R551H and R551C despite of occurring NAD+-binding and hydrolysis	Pseudomonas aeruginosa	?	-	?
NAD+ + essential ribosomal elongation factor 2	transfer of ADP-ribose moiety (oxocarbenium ion) of NAD+ onto N3 of diphthamide imidazole of essential ribosomal elongation factor 2 (eEF2)	Pseudomonas aeruginosa	nicotinamide + H+ + ADP-ribosylated essential ribosomal elongation factor 2	-	?
NAD+ + essential ribosomal elongation factor 2	essential ribosomal elongation factor 2: eEF2, 2-step reaction by concerted GH and ADPRT activity, transition-state model	Pseudomonas aeruginosa	nicotinamide + H+ + ADP-ribosylated essential ribosomal elongation factor 2	-	?

Synonyms

Synonyms	Comment	Organism
ExoA	-	Pseudomonas aeruginosa
ExoA(c)	-	Pseudomonas aeruginosa
exotoxin A	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
additional information	-	NAD+	0.0062-0.13/min, wild-type GH activity, in the absence of eEF2, pH 7.9, 25°C, 60 min	Pseudomonas aeruginosa
additional information	-	NAD+	0.125 +/-0.007/min (a), wild-type GH activity, in the absence of eEF2, pH 7.9, 25°C, 60 min	Pseudomonas aeruginosa
additional information	-	NAD+	0.130 +/-0.012/min (b), wild-type GH activity, in the absence of eEF2, pH 7.9, 25°C, 60 min	Pseudomonas aeruginosa
additional information	-	NAD+	1306 +/-121/min (a), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec	Pseudomonas aeruginosa
additional information	-	NAD+	1495 +/-224/min (b), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec	Pseudomonas aeruginosa
additional information	-	NAD+	628 +/-8/min (B), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec	Pseudomonas aeruginosa
additional information	-	NAD+	746 +/-18/min (A), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec	Pseudomonas aeruginosa
additional information	-	NAD+	847 +/-21/min (C), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec	Pseudomonas aeruginosa
additional information	-	NAD+	900 +/-20/min (D), wild-type ADPRT activity, 12 microM eEF2, pH 7.9, 25°C, 300 sec	Pseudomonas aeruginosa
additional information	-	NAD+	double mutant E546A/R551A, kcat = 0.0001 +/-0.0001, relative to wild-type ADPRT activity (C)	Pseudomonas aeruginosa
additional information	-	NAD+	double mutant E546A/R551A, kcat = 0.84 +/-0.04, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	double mutant E546D/R551K, kcat = 0.0009 +/-0.0013, relative to wild-type ADPRT activity (C)	Pseudomonas aeruginosa
additional information	-	NAD+	double mutant E546D/R551K, kcat = 0.42 +/-0.0002, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	double mutant E546R/R551E, kcat = 0.00001 +/-0.000002, relative to wild-type ADPRT activity (C)	Pseudomonas aeruginosa
additional information	-	NAD+	double mutant E546R/R551E, kcat = 0.38 +/-0.01, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant D461A, kcat = 1.001 +/-0.091, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant D461A, kcat = 1.076 +/-0.041, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant D463A, kcat = 1.148 +/-0.038, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant D463A, kcat = 1.270 +/-0.204, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546A, kcat = 0.0012 +/-0.00002, relative to wild-type ADPRT activity (A)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546A, kcat = 0.24 +/-0.008, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546D, kcat = 0.00025 +/-0.00005, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546D, kcat = 0.45 +/-0.002, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546F, kcat = 0.007 +/-0.0002, relative to wild-type ADPRT activity (D)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546F, kcat = 0.69 +/-0.05, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546H, kcat = 0.0012 +/-0.0002, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546H, kcat = 0.59 +/-0.009, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546N, kcat = 0.0010 +/-0.00009, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546N, kcat = 0.67 +/-0.03, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546Q, kcat = 0.011 +/-0.002, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E546Q, kcat = 0.80 +/-0.04, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E547A, kcat = 0.795 +/-0.077, relative to wild-type ADPRT activity (A)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E547A, kcat = 0.83 +/-0.14, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E548A, kcat = 0.76 +/-0.01, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E548A, kcat = 1.669 +/-0.298, relative to wild-type ADPRT activity (A)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E553A, kcat = 0.0015 +/-0.00005, relative to wild-type ADPRT activity (A)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant E553A, kcat = 0.07 +/-0.009, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G453A, kcat = 0.290 +/-0.040, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G453A, kcat = 0.331 +/-0.019, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G454A, kcat = 0.031 +/-0.003, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G454A, kcat = 0.046 +/-0.008, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G549A, kcat = 0.30 +/-0.005, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G549A, kcat = 0.770 +/-0.076, relative to wild-type ADPRT activity (A)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G550A, kcat = 0.41 +/-0.04, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant G550A, kcat = 0.562 +/-0.166, relative to wild-type ADPRT activity (A)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant L462A, kcat = 0.268 +/-0.041, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant L462A, kcat = 0.955 +/-0.015, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant L552A, kcat = 0.58 +/-0.002, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant L552A, kcat = 2.365 +/-0.527, relative to wild-type ADPRT activity (A)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant Q460A, kcat = 0.115 +/-0.010, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant Q460A, kcat = 0.622 +/-0.014, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R456A, kcat = 0.230 +/-0.010, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R456A, kcat = 0.239 +/-0.034, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458A, kcat = 0.132 +/-0.031, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458A, kcat = 0.249 +/-0.021, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458H, kcat = 0.018 +/-0.003, relative to wild-type GH activity (b)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458H, kcat = 0.019 +/-0.007, relative to wild-type ADPRT activity (b)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458K, kcat = 0.428 +/-0.033, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458K, kcat = 0.543 +/-0.155, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458Q, kcat = 0.650 +/-0.130, relative to wild-type ADPRT activity (b)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458Q, kcat = 0.991 +/-0.017, relative to wild-type GH activity (b)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458W, kcat = 0.379 +/-0.043, relative to wild-type ADPRT activity (b)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R458W, kcat = 0.438 +/-0.040, relative to wild-type GH activity (b)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551A, kcat = 0.32 +/-0.005, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551A, kcat = 0.380 +/-0.024, relative to wild-type ADPRT activity (A)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551C, kcat = ~0, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551C, kcat = 0.10 +/-0.006, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551E, kcat = 0.011 +/-0.005, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551E, kcat = 0.09 +/-0.001, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551H, kcat = ~0, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551H, kcat = 0.41 +/-0.002, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551K, kcat = 0.317 +/-0.024, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551K, kcat = 0.69 +/-0.03, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551Q, kcat = 0.185 +/-0.014, relative to wild-type ADPRT activity (B)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant R551Q, kcat = 0.38 +/-0.01, relative to wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	mutant S459A, kcat = 1.119 +/-0.054, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant S459A, kcat = 1.256 +/-0.144, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant V455A, kcat = 0.241 +/-0.032, relative to wild-type ADPRT activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	mutant V455A, kcat = 0.254 +/-0.001, relative to wild-type GH activity (a)	Pseudomonas aeruginosa
additional information	-	NAD+	relative kcat = 1.00 +/-0.02, wild-type GH activity	Pseudomonas aeruginosa
additional information	-	NAD+	relative kcat = 1.00 +/-0.05, wild-type GH activity (a) and (b)	Pseudomonas aeruginosa
additional information	-	NAD+	relative kcat = 1.00 +/-0.09, wild-type ADPRT activity (A)-(D), and (a) and (b)	Pseudomonas aeruginosa