BRENDA - Enzyme Database show
show all sequences of 2.4.2.36

The exotoxin P. aeruginosa: a proenzyme having an unusual mode of activation

Leppla, S.H.; Martin, O.C.; Muehl, L.A.; Biochem. Biophys. Res. Commun. 81, 532-538 (1978)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
cysteine
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
dithiothreitol
enhances activity together with urea
Pseudomonas aeruginosa
guanidine hydrochloride
enhances activity together with dithiothreitol, cysteine, 2-mercaptoethanol or sulfite
Pseudomonas aeruginosa
additional information
exotoxin is synthesized in a catalytically inactive, proenzyme form, unfolding of the toxin molecule may cause activation
Pseudomonas aeruginosa
SDS
enhances activity together with dithiothreitol, cysteine, 2-mercaptoethanol or sulfite
Pseudomonas aeruginosa
sulfite
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
Urea
enhances activity together with dithiothreitol
Pseudomonas aeruginosa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
exotoxin A
Pseudomonas aeruginosa
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + elongation factor 2
Pseudomonas aeruginosa
NAD-glycohydrolase activity without acceptor substrate
nicotinamide + ADPribose-elongation factor 2
-
Pseudomonas aeruginosa
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture supernatant
-
Pseudomonas aeruginosa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + elongation factor 2
NAD-glycohydrolase activity without acceptor substrate
489800
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
489800
Pseudomonas aeruginosa
-
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
cysteine
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
dithiothreitol
enhances activity together with urea
Pseudomonas aeruginosa
guanidine hydrochloride
enhances activity together with dithiothreitol, cysteine, 2-mercaptoethanol or sulfite
Pseudomonas aeruginosa
additional information
exotoxin is synthesized in a catalytically inactive, proenzyme form, unfolding of the toxin molecule may cause activation
Pseudomonas aeruginosa
SDS
enhances activity together with dithiothreitol, cysteine, 2-mercaptoethanol or sulfite
Pseudomonas aeruginosa
sulfite
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
Urea
enhances activity together with dithiothreitol
Pseudomonas aeruginosa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
exotoxin A
Pseudomonas aeruginosa
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + elongation factor 2
Pseudomonas aeruginosa
NAD-glycohydrolase activity without acceptor substrate
nicotinamide + ADPribose-elongation factor 2
-
Pseudomonas aeruginosa
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture supernatant
-
Pseudomonas aeruginosa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + elongation factor 2
NAD-glycohydrolase activity without acceptor substrate
489800
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
489800
Pseudomonas aeruginosa
-
Other publictions for EC 2.4.2.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Lugo
The father, son and cholix tox ...
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7
2757-2772
2015
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5
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2
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6
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5
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2
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2
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722762
Fieldhouse
The 1.8 A cholix toxin crystal ...
Vibrio cholerae
J. Biol. Chem.
287
21176-21188
2012
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1
8
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1
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2
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1
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1
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699495
Jang
Improved purification process ...
Vibrio cholerae, Vibrio cholerae 569B
J. Microbiol. Biotechnol.
19
108-112
2009
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1
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3
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701050
Gong
Cloning, expression, purificat ...
Vibrio cholerae
Protein Expr. Purif.
191
191-197
2009
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1
1
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1
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1
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5
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1
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690825
Zhang
The role of the diphthamide-co ...
Pseudomonas aeruginosa
Biochem. J.
413
163-174
2008
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1
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2
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3
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1
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692077
Jorgensen
The nature and character of th ...
Pseudomonas aeruginosa
EMBO Rep.
9
802-809
2008
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1
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1
33
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1
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2
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4
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4
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1
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1
33
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4
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1
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696625
Zhang
Design, synthesis, and evaluat ...
Vibrio cholerae
Bioorg. Med. Chem. Lett.
18
3724-3727
2008
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19
2
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1
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1
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1
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2
1
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8
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8
19
8
2
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1
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2
1
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698219
Feng
Investigation of the role of c ...
Vibrio cholerae
Immunol. Invest.
37
782-797
2008
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2
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1
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658054
Armstrong
Toward the elucidation of the ...
Pseudomonas aeruginosa
Biochemistry
43
183-194
2004
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489798
Lee
Cellular ADP-ribosyltransferas ...
Bos taurus, Mesocricetus auratus, Pseudomonas aeruginosa
Proc. Natl. Acad. Sci. USA
81
2703-2707
1984
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489799
Sanai
Proteolytic cleavage of exotox ...
Pseudomonas aeruginosa
FEBS Lett.
120
131-134
1980
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489800
Leppla
The exotoxin P. aeruginosa: a ...
Pseudomonas aeruginosa
Biochem. Biophys. Res. Commun.
81
532-538
1978
8
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