Data extracted from this reference:
Inhibitors
Cytoplasmic extract of pyBHK-cells
not fragment A
Mesocricetus auratus
histamine
250 mM, almost complete inhibition
Mesocricetus auratus
additional information
cellular ADPribosyltransferase is not inhibited by anti-fragment A-antiserum
Mesocricetus auratus
additional information
fragment A and toxin A are not inhibited by histamine
Pseudomonas aeruginosa
Localization
extracellular
Mesocricetus auratus
extracellular
fragment A of diphtheria toxin
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
NAD+ + elongation factor 2
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
Pseudomonas aeruginosa
r
NAD+ + elongation factor 2
Bos taurus
liver enzyme
nicotinamide + ADPribose-elongation factor 2
Bos taurus
?
NAD+ + elongation factor 2
Mesocricetus auratus
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
Mesocricetus auratus
r
Organism
Mesocricetus auratus
baby
Source Tissue
kidney
polyoma virus transformed baby hamster kidney cells, i.e. pyBHK-cells
Mesocricetus auratus
Substrates and Products (Substrate)
NAD+ + elongation factor 2
489798
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
489798
Pseudomonas aeruginosa
r
NAD+ + elongation factor 2
liver enzyme
489798
Bos taurus
nicotinamide + ADPribose-elongation factor 2
489798
Bos taurus
?
NAD+ + elongation factor 2
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
489798
Mesocricetus auratus
nicotinamide + ADPribose-elongation factor 2
489798
Mesocricetus auratus
r
Temperature Optimum [°C]
22
assay at
Mesocricetus auratus
pH Optimum
6.6
assay at, nicotinamide + ADPribose-elongation factor 2
Mesocricetus auratus
8
assay at, NAD+ + elongation factor 2
Mesocricetus auratus
Inhibitors (protein specific)
Cytoplasmic extract of pyBHK-cells
not fragment A
Mesocricetus auratus
histamine
250 mM, almost complete inhibition
Mesocricetus auratus
additional information
cellular ADPribosyltransferase is not inhibited by anti-fragment A-antiserum
Mesocricetus auratus
additional information
fragment A and toxin A are not inhibited by histamine
Pseudomonas aeruginosa
Localization (protein specific)
extracellular
Mesocricetus auratus
extracellular
fragment A of diphtheria toxin
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
NAD+ + elongation factor 2
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
Pseudomonas aeruginosa
r
NAD+ + elongation factor 2
Bos taurus
liver enzyme
nicotinamide + ADPribose-elongation factor 2
Bos taurus
?
NAD+ + elongation factor 2
Mesocricetus auratus
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
Mesocricetus auratus
r
Source Tissue (protein specific)
kidney
polyoma virus transformed baby hamster kidney cells, i.e. pyBHK-cells
Mesocricetus auratus
Substrates and Products (Substrate) (protein specific)
NAD+ + elongation factor 2
489798
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
489798
Pseudomonas aeruginosa
r
NAD+ + elongation factor 2
liver enzyme
489798
Bos taurus
nicotinamide + ADPribose-elongation factor 2
489798
Bos taurus
?
NAD+ + elongation factor 2
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
489798
Mesocricetus auratus
nicotinamide + ADPribose-elongation factor 2
489798
Mesocricetus auratus
r
Temperature Optimum [°C] (protein specific)
22
assay at
Mesocricetus auratus
pH Optimum (protein specific)
6.6
assay at, nicotinamide + ADPribose-elongation factor 2
Mesocricetus auratus
8
assay at, NAD+ + elongation factor 2
Mesocricetus auratus
Other publictions for EC 2.4.2.36
737282
Lugo
The father, son and cholix tox ...
Pseudomonas aeruginosa, Vibrio cholerae
Toxins
7
2757-2772
2015
-
-
-
-
-
-
5
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
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-
2
-
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-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722762
Fieldhouse
The 1.8 A cholix toxin crystal ...
Vibrio cholerae serotype O1
J. Biol. Chem.
287
21176-21188
2012
-
-
-
1
8
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
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1
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-
1
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-
1
8
-
1
1
-
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-
-
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-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
699495
Jang
Improved purification process ...
Vibrio cholerae serotype O1, Vibrio cholerae serotype O1 569B
J. Microbiol. Biotechnol.
19
108-112
2009
-
1
-
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-
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-
2
-
-
1
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-
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1
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1
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1
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-
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-
-
-
-
-
-
701050
Gong
Cloning, expression, purificat ...
Vibrio cholerae serotype O1
Protein Expr. Purif.
191
191-197
2009
-
1
1
-
1
-
-
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
690825
Zhang
The role of the diphthamide-co ...
Pseudomonas aeruginosa
Biochem. J.
413
163-174
2008
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
1
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
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-
-
3
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
692077
Jorgensen
The nature and character of th ...
Pseudomonas aeruginosa
EMBO Rep.
9
802-809
2008
-
1
-
1
33
-
-
1
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
3
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
33
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
696625
Zhang
Design, synthesis, and evaluat ...
Vibrio cholerae serotype O1
Bioorg. Med. Chem. Lett.
18
3724-3727
2008
-
-
-
-
-
-
19
2
-
-
-
1
-
1
-
1
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
8
-
8
-
-
-
-
-
-
-
8
19
8
2
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698219
Feng
Investigation of the role of c ...
Vibrio cholerae serotype O1
Immunol. Invest.
37
782-797
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658054
Armstrong
Toward the elucidation of the ...
Pseudomonas aeruginosa
Biochemistry
43
183-194
2004
-
-
-
-
-
-
1
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
489798
Lee
Cellular ADP-ribosyltransferas ...
Bos taurus, Mesocricetus auratus, Pseudomonas aeruginosa
Proc. Natl. Acad. Sci. USA
81
2703-2707
1984
-
-
-
-
-
-
4
-
3
-
-
3
-
5
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
3
-
-
3
-
-
-
-
-
2
-
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
489799
Sanai
Proteolytic cleavage of exotox ...
Pseudomonas aeruginosa
FEBS Lett.
120
131-134
1980
-
-
-
-
-
-
1
-
1
-
1
1
-
3
-
-
1
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
1
-
-
-
1
-
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489800
Leppla
The exotoxin P. aeruginosa: a ...
Pseudomonas aeruginosa
Biochem. Biophys. Res. Commun.
81
532-538
1978
8
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-