Cellular ADP-ribosyltransferase with the same mechanism of action as diphtheria toxin and Pseudomonas toxin A

Lee, H.; Iglewski, W.J.; Proc. Natl. Acad. Sci. USA 81, 2703-2707 (1984)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Cytoplasmic extract of pyBHK-cells

not fragment A

Mesocricetus auratus

histamine

250 mM, almost complete inhibition

Mesocricetus auratus

additional information

cellular ADPribosyltransferase is not inhibited by anti-fragment A-antiserum

Mesocricetus auratus

additional information

fragment A and toxin A are not inhibited by histamine

Pseudomonas aeruginosa

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

NAD+ + elongation factor 2

Pseudomonas aeruginosa

-

nicotinamide + ADPribose-elongation factor 2

-

Pseudomonas aeruginosa

r

NAD+ + elongation factor 2

Bos taurus

liver enzyme

nicotinamide + ADPribose-elongation factor 2

-

Bos taurus

?

NAD+ + elongation factor 2

Mesocricetus auratus

i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa

nicotinamide + ADPribose-elongation factor 2

-

Mesocricetus auratus

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

NAD+ + elongation factor 2

-

489798

Pseudomonas aeruginosa

nicotinamide + ADPribose-elongation factor 2

-

489798

Pseudomonas aeruginosa

r

NAD+ + elongation factor 2

liver enzyme

489798

Bos taurus

nicotinamide + ADPribose-elongation factor 2

-

489798

Bos taurus

?

NAD+ + elongation factor 2

i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa

489798

Mesocricetus auratus

nicotinamide + ADPribose-elongation factor 2

-

489798

Mesocricetus auratus

r

Inhibitors

Commentary

Organism

Structure

Cytoplasmic extract of pyBHK-cells

not fragment A

Mesocricetus auratus

histamine

250 mM, almost complete inhibition

Mesocricetus auratus

additional information

cellular ADPribosyltransferase is not inhibited by anti-fragment A-antiserum

Mesocricetus auratus

additional information

fragment A and toxin A are not inhibited by histamine

Pseudomonas aeruginosa

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

NAD+ + elongation factor 2

Pseudomonas aeruginosa

-

nicotinamide + ADPribose-elongation factor 2

-

Pseudomonas aeruginosa

r

NAD+ + elongation factor 2

Bos taurus

liver enzyme

nicotinamide + ADPribose-elongation factor 2

-

Bos taurus

?

NAD+ + elongation factor 2

Mesocricetus auratus

i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa

nicotinamide + ADPribose-elongation factor 2

-

Mesocricetus auratus

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

NAD+ + elongation factor 2

-

489798

Pseudomonas aeruginosa

nicotinamide + ADPribose-elongation factor 2

-

489798

Pseudomonas aeruginosa

r

NAD+ + elongation factor 2

liver enzyme

489798

Bos taurus

nicotinamide + ADPribose-elongation factor 2

-

489798

Bos taurus

?

NAD+ + elongation factor 2

i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa

489798

Mesocricetus auratus

nicotinamide + ADPribose-elongation factor 2

-

489798

Mesocricetus auratus

r

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

737282

Lugo

The father, son and cholix tox ...

Pseudomonas aeruginosa, Vibrio cholerae

Toxins

7

2757-2772

2015

-

-

-

-

-

-

5

-

-

-

-

2

-

5

-

-

-

-

-

-

-

-

2

-

6

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

-

-

2

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

722762

Fieldhouse

The 1.8 A cholix toxin crystal ...

Vibrio cholerae serotype O1

J. Biol. Chem.

287

21176-21188

2012

-

-

-

1

8

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

1

1

-

-

-

1

-

-

-

-

-

1

-

-

-

-

1

8

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

699495

Jang

Improved purification process ...

Vibrio cholerae serotype O1, Vibrio cholerae serotype O1 569B

J. Microbiol. Biotechnol.

19

108-112

2009

-

1

-

-

-

-

-

-

-

-

-

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-

2

-

-

1

-

-

-

-

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-

1

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

701050

Gong

Cloning, expression, purificat ...

Vibrio cholerae serotype O1

Protein Expr. Purif.

191

191-197

2009

-

1

1

-

1

-

-

-

-

-

1

-

-

5

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

690825

Zhang

The role of the diphthamide-co ...

Pseudomonas aeruginosa

Biochem. J.

413

163-174

2008

-

-

1

-

-

-

-

-

-

-

-

2

-

3

-

-

1

-

-

-

-

-

3

-

2

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

2

-

-

-

1

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

692077

Jorgensen

The nature and character of th ...

Pseudomonas aeruginosa

EMBO Rep.

9

802-809

2008

-

1

-

1

33

-

-

1

-

-

-

2

-

4

-

-

-

-

-

-

-

-

4

-

3

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

33

-

-

-

-

1

-

-

-

2

-

-

-

-

-

-

-

-

4

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

696625

Zhang

Design, synthesis, and evaluat ...

Vibrio cholerae serotype O1

Bioorg. Med. Chem. Lett.

18

3724-3727

2008

-

-

-

-

-

-

19

2

-

-

-

1

-

1

-

1

-

-

-

-

-

-

2

1

1

-

-

-

-

-

-

-

-

8

-

8

-

-

-

-

-

-

-

8

19

8

2

-

-

-

1

-

-

1

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

698219

Feng

Investigation of the role of c ...

Vibrio cholerae serotype O1

Immunol. Invest.

37

782-797

2008

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

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-

-

-

-

-

-

-

-

-

-

-

-

658054

Armstrong

Toward the elucidation of the ...

Pseudomonas aeruginosa

Biochemistry

43

183-194

2004

-

-

-

-

-

-

1

2

-

-

-

-

-

3

-

-

-

-

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-

-

-

1

-

1

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

2

-

-

-

-

-

-

-

-

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-

-

-

1

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

489798

Lee

Cellular ADP-ribosyltransferas ...

Bos taurus, Mesocricetus auratus, Pseudomonas aeruginosa

Proc. Natl. Acad. Sci. USA

81

2703-2707

1984

-

-

-

-

-

-

4

-

3

-

-

3

-

5

-

-

-

-

-

2

-

-

3

-

-

1

-

-

-

2

-

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-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

3

-

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3

-

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2

-

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3

-

1

-

-

-

2

-

-

-

-

-

-

-

-

-

489799

Sanai

Proteolytic cleavage of exotox ...

Pseudomonas aeruginosa

FEBS Lett.

120

131-134

1980

-

-

-

-

-

-

1

-

1

-

1

1

-

3

-

-

1

-

-

1

-

-

1

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

1

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1

1

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1

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1

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1

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

489800

Leppla

The exotoxin P. aeruginosa: a ...

Pseudomonas aeruginosa

Biochem. Biophys. Res. Commun.

81

532-538

1978

8

-

-

-

-

-

-

-

1

-

-

1

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

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-

-

8

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

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-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

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-

-

-

-