BRENDA - Enzyme Database show
show all sequences of 2.4.2.36

Cellular ADP-ribosyltransferase with the same mechanism of action as diphtheria toxin and Pseudomonas toxin A

Lee, H.; Iglewski, W.J.; Proc. Natl. Acad. Sci. USA 81, 2703-2707 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cytoplasmic extract of pyBHK-cells
not fragment A
Mesocricetus auratus
histamine
250 mM, almost complete inhibition
Mesocricetus auratus
additional information
cellular ADPribosyltransferase is not inhibited by anti-fragment A-antiserum
Mesocricetus auratus
additional information
fragment A and toxin A are not inhibited by histamine
Pseudomonas aeruginosa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Bos taurus
-
-
extracellular
-
Mesocricetus auratus
-
-
extracellular
fragment A of diphtheria toxin
Pseudomonas aeruginosa
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + elongation factor 2
Pseudomonas aeruginosa
-
nicotinamide + ADPribose-elongation factor 2
-
Pseudomonas aeruginosa
r
NAD+ + elongation factor 2
Bos taurus
liver enzyme
nicotinamide + ADPribose-elongation factor 2
-
Bos taurus
?
NAD+ + elongation factor 2
Mesocricetus auratus
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
Mesocricetus auratus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Mesocricetus auratus
-
baby
-
Pseudomonas aeruginosa
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
polyoma virus transformed baby hamster kidney cells, i.e. pyBHK-cells
Mesocricetus auratus
-
liver
-
Bos taurus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + elongation factor 2
-
489798
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
489798
Pseudomonas aeruginosa
r
NAD+ + elongation factor 2
liver enzyme
489798
Bos taurus
nicotinamide + ADPribose-elongation factor 2
-
489798
Bos taurus
?
NAD+ + elongation factor 2
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
489798
Mesocricetus auratus
nicotinamide + ADPribose-elongation factor 2
-
489798
Mesocricetus auratus
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at
Mesocricetus auratus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
assay at, nicotinamide + ADPribose-elongation factor 2
Mesocricetus auratus
8
-
assay at, NAD+ + elongation factor 2
Mesocricetus auratus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cytoplasmic extract of pyBHK-cells
not fragment A
Mesocricetus auratus
histamine
250 mM, almost complete inhibition
Mesocricetus auratus
additional information
cellular ADPribosyltransferase is not inhibited by anti-fragment A-antiserum
Mesocricetus auratus
additional information
fragment A and toxin A are not inhibited by histamine
Pseudomonas aeruginosa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Bos taurus
-
-
extracellular
-
Mesocricetus auratus
-
-
extracellular
fragment A of diphtheria toxin
Pseudomonas aeruginosa
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + elongation factor 2
Pseudomonas aeruginosa
-
nicotinamide + ADPribose-elongation factor 2
-
Pseudomonas aeruginosa
r
NAD+ + elongation factor 2
Bos taurus
liver enzyme
nicotinamide + ADPribose-elongation factor 2
-
Bos taurus
?
NAD+ + elongation factor 2
Mesocricetus auratus
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
Mesocricetus auratus
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
polyoma virus transformed baby hamster kidney cells, i.e. pyBHK-cells
Mesocricetus auratus
-
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + elongation factor 2
-
489798
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
489798
Pseudomonas aeruginosa
r
NAD+ + elongation factor 2
liver enzyme
489798
Bos taurus
nicotinamide + ADPribose-elongation factor 2
-
489798
Bos taurus
?
NAD+ + elongation factor 2
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
489798
Mesocricetus auratus
nicotinamide + ADPribose-elongation factor 2
-
489798
Mesocricetus auratus
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at
Mesocricetus auratus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
-
assay at, nicotinamide + ADPribose-elongation factor 2
Mesocricetus auratus
8
-
assay at, NAD+ + elongation factor 2
Mesocricetus auratus
Other publictions for EC 2.4.2.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Lugo
The father, son and cholix tox ...
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7
2757-2772
2015
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5
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2
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6
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2
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5
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2
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2
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722762
Fieldhouse
The 1.8 A cholix toxin crystal ...
Vibrio cholerae
J. Biol. Chem.
287
21176-21188
2012
-
-
-
1
8
-
1
-
-
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2
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1
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1
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1
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1
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1
8
-
1
1
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1
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1
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1
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699495
Jang
Improved purification process ...
Vibrio cholerae, Vibrio cholerae 569B
J. Microbiol. Biotechnol.
19
108-112
2009
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1
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3
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1
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701050
Gong
Cloning, expression, purificat ...
Vibrio cholerae
Protein Expr. Purif.
191
191-197
2009
-
1
1
-
1
-
-
-
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1
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5
-
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1
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-
-
-
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1
1
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1
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1
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1
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690825
Zhang
The role of the diphthamide-co ...
Pseudomonas aeruginosa
Biochem. J.
413
163-174
2008
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-
1
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2
-
3
-
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1
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3
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1
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1
1
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2
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1
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3
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692077
Jorgensen
The nature and character of th ...
Pseudomonas aeruginosa
EMBO Rep.
9
802-809
2008
-
1
-
1
33
-
-
1
-
-
-
2
-
4
-
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4
-
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-
1
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1
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1
33
-
-
-
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1
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2
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-
-
4
-
-
-
-
1
-
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696625
Zhang
Design, synthesis, and evaluat ...
Vibrio cholerae
Bioorg. Med. Chem. Lett.
18
3724-3727
2008
-
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-
19
2
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1
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1
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1
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2
1
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8
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8
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8
19
8
2
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1
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1
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2
1
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698219
Feng
Investigation of the role of c ...
Vibrio cholerae
Immunol. Invest.
37
782-797
2008
-
-
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2
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1
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1
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658054
Armstrong
Toward the elucidation of the ...
Pseudomonas aeruginosa
Biochemistry
43
183-194
2004
-
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1
2
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3
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1
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2
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1
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2
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-
489798
Lee
Cellular ADP-ribosyltransferas ...
Bos taurus, Mesocricetus auratus, Pseudomonas aeruginosa
Proc. Natl. Acad. Sci. USA
81
2703-2707
1984
-
-
-
-
-
-
4
-
3
-
-
3
-
5
-
-
-
-
-
2
-
-
3
-
1
-
-
-
2
-
-
-
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-
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-
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4
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3
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3
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2
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3
-
1
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-
2
-
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-
489799
Sanai
Proteolytic cleavage of exotox ...
Pseudomonas aeruginosa
FEBS Lett.
120
131-134
1980
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1
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1
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1
1
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3
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1
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1
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1
1
1
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1
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1
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1
1
1
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489800
Leppla
The exotoxin P. aeruginosa: a ...
Pseudomonas aeruginosa
Biochem. Biophys. Res. Commun.
81
532-538
1978
8
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1
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1
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1
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1
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1
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8
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1
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1
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1
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1
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