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Literature summary for 2.4.2.31 extracted from

  • Jucovic, M.; Walters, F.S.; Warren, G.W.; Palekar, N.V.; Chen, J.S.
    From enzyme to zymogen: engineering Vip2, an ADP-ribosyltransferase from Bacillus cereus, for conditional toxicity (2008), Protein Eng. Des. Sel., 21, 631-638.
    View publication on PubMed
Search for more literature for 2.4.2.31

Application

Application Comment Organism
agriculture engineering strategy for the creation of a plant-tolerated, zymogen-like form of an otherwise toxic protein. Engineering of a random propeptide library at the C-terminal end of ADP-ribosyltranferase Vip2 and selecting for malfunctional enzyme variants in yeast leads to a proenzyme proVip2 which possesses reduced enzymatic activity as compared with the wild-type Vip2 protein, but remains a potent toxin toward rootworm larvae. The zymogenized Vip2 can be proteolytically activated by rootworm digestive enzyme machinery Bacillus cereus

Protein Variants

Protein Variants Comment Organism
additional information engineering strategy for the creation of a plant-tolerated, zymogen-like form of an otherwise toxic protein. Engineering of a random propeptide library at the C-terminal end of ADP-ribosyltranferase Vip2 and selecting for malfunctional enzyme variants in yeast leads to a proenzyme proVip2 which possesses reduced enzymatic activity as compared with the wild-type Vip2 protein, but remains a potent toxin toward rootworm larvae. The zymogenized Vip2 can be proteolytically activated by rootworm digestive enzyme machinery Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
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Synonyms

Synonyms Comment Organism
VIP2
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 Bacillus cereus