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Literature summary for 2.4.2.31 extracted from

  • Skorko, R.; Zillig, W.; Rohrer, H.; Mailhammer, R.
    Purification and properties of the NAD+: protein ADP-ribosyltransferase responsible for the T4-phage-induced modification of the alpha subunit of DNA-dependent RNA polymerase of Escherichia coli (1977), Eur. J. Biochem., 79, 55-66.
    View publication on PubMed

General Stability

General Stability Organism
unstable during dialysis, loss of activity can be strongly reduced by using buffers containing 40% glycerol Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
AMP 7.8 mM, decreases activity to 78% of the uninhibited control Tequatrovirus T4
nicotinamide
-
Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0143
-
NAD+
-
Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
gel filtration Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
Escherichia coli B/r infected with phage T4
-

Purification (Commentary)

Purification (Comment) Organism
-
Tequatrovirus T4

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polyarginine + NAD+
-
Tequatrovirus T4 nicotinamide + ?
-
ir
RNA polymerase + NAD+ alpha-subunit of RNA polymerase Tequatrovirus T4 additional information the amino acid carrying the ADP-ribosyl residue appears to be arginine ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
Tequatrovirus T4

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 37 20°C: optimum, 37°C: about 35% of maximal activity Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Tequatrovirus T4

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6: about 50% of maximal activity, pH 9: about 80% of maximal activity Tequatrovirus T4

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4
-
nicotinamide
-
Tequatrovirus T4