General Stability | Organism |
---|---|
unstable during dialysis, loss of activity can be strongly reduced by using buffers containing 40% glycerol | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 7.8 mM, decreases activity to 78% of the uninhibited control | Tequatrovirus T4 | |
nicotinamide | - |
Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0143 | - |
NAD+ | - |
Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
gel filtration | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
Escherichia coli B/r infected with phage T4 | - |
Purification (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polyarginine + NAD+ | - |
Tequatrovirus T4 | nicotinamide + ? | - |
ir | |
RNA polymerase + NAD+ | alpha-subunit of RNA polymerase | Tequatrovirus T4 | additional information | the amino acid carrying the ADP-ribosyl residue appears to be arginine | ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Tequatrovirus T4 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 37 | 20°C: optimum, 37°C: about 35% of maximal activity | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Tequatrovirus T4 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6: about 50% of maximal activity, pH 9: about 80% of maximal activity | Tequatrovirus T4 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
nicotinamide | - |
Tequatrovirus T4 |