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Literature summary for 2.4.2.31 extracted from

  • Moss, J.; Vaughan, M.
    NAD: arginine mono-ADP-ribosyltransferases from animal cells (1984), Methods Enzymol., 106, 430-437.
    View publication on PubMed
Search for more literature for 2.4.2.31

Activating Compound

Activating Compound Comment Organism Structure
Br- activates NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
Cl- activates NAD:arginine ADP-ribosyltransferase A, NaCl is maximally effective at 250 mM Meleagris gallopavo
F- activates NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
histone increases activity in absence of salt Meleagris gallopavo
lysophosphatidylcholine increases activity in the order of declining effectiveness C18, C16, C14, C12, C10, C8, NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
PO43- activates NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
SCN- activates NAD:arginine ADP-ribosyltransferase A, NaSCN is maximally effective at 100 mM Meleagris gallopavo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 NAD+ NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
0.036
-
 NAD+ NAD:arginine ADP-ribosyltransferase B Meleagris gallopavo
1.3
-
 Arginine methyl ester in presence of 250 mM NaCl, NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
3
-
 Arginine methyl ester NAD:arginine ADP-ribosyltransferase B Meleagris gallopavo

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25300
-
 NAD:arginine ADP-ribosyltransferase A, gel filtration Meleagris gallopavo
28000
-
 1 * 28000, NAD:arginine ADP-ribosyltransferase A, SDS-PAGE Meleagris gallopavo
32000
-
 x * 32000, NAD:arginine ADP-ribosyltransferase B, SDS-PAGE Meleagris gallopavo

Organism

Organism UniProt Comment Textmining
Meleagris gallopavo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
NAD:arginine ADP-ribosyltransferase B Meleagris gallopavo

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Meleagris gallopavo
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
 NAD:arginine ADP-ribosyltransferase B Meleagris gallopavo
353
-
 NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo

Storage Stability

Storage Stability Organism
0-4°C, 50 mM sodium phosphate, pH 7.1, 100 mM NaCl, NAD:arginine ADP-ribosyltransferase B, stable Meleagris gallopavo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine methyl ester + NADP+
-
 Meleagris gallopavo nicotinamide + N2-(2'-phospho-ADP-D-ribosyl)-L-arginine
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, NAD:arginine ADP-ribosyltransferase B, SDS-PAGE Meleagris gallopavo
monomer 1 * 28000, NAD:arginine ADP-ribosyltransferase A, SDS-PAGE Meleagris gallopavo

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is preferred over NADP+, NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo
NADP+ NAD+ is preferred over NADP+, NAD:arginine ADP-ribosyltransferase A Meleagris gallopavo