Literature summary for 2.4.2.30 extracted from

Simon, N.; Vergis, J.; Ebrahimi, A.; Ventura, C.; O'Brien, A.; Barbieri, J.
Host cell cytotoxicity and cytoskeleton disruption by CerADPr, an ADP-ribosyltransferase of Bacillus cereus G9241 (2013), Biochemistry, 52, 2309-2318.

Search for more literature for 2.4.2.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bacillus cereus

Protein Variants

Protein Variants	Comment	Organism
E431D	inactive	Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29451	-	4 * 29451, calculated from amino acid sequence	Bacillus cereus
30000	-	4 * 30000, SDS-PAGE	Bacillus cereus
120000	-	gel filtration	Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	-	-	-
Bacillus cereus G9241	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ affinity resin column chromatography and Sephacryl S200 gel filtration	Bacillus cereus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + (ADP-D-ribosyl)n-biotin	-	Bacillus cereus	nicotinamide + (ADP-D-ribosyl)n+1-biotin + H+	-	?
NAD+ + (ADP-D-ribosyl)n-biotin	-	Bacillus cereus G9241	nicotinamide + (ADP-D-ribosyl)n+1-biotin + H+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 30000, SDS-PAGE	Bacillus cereus
homotetramer	4 * 29451, calculated from amino acid sequence	Bacillus cereus

Synonyms

Synonyms	Comment	Organism
ADP-ribosyltransferase	-	Bacillus cereus
ADPr	-	Bacillus cereus

General Information

General Information	Comment	Organism
physiological function	the enzyme disrupts host cytoskeletal signaling	Bacillus cereus

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Release 2023.1 (January 2023)