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Literature summary for 2.4.2.30 extracted from

  • Shniffer, A.; Visschedyk, D.D.; Ravulapalli, R.; Suarez, G.; Turgeon, Z.J.; Petrie, A.A.; Chopra, A.K.; Merrill, A.R.
    Characterization of an actin-targeting ADP-ribosyltransferase from Aeromonas hydrophila (2012), J. Biol. Chem., 287, 37030-37041.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.30

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Aeromonas hydrophila

Protein Variants

Protein Variants Comment Organism
E213A kcat highly decreased compared to wild-type, Km similar to wild-type Aeromonas hydrophila
E215A kcat highly decreased compared to wild-type, Km (ADP-D-ribosyl)n-actin similar to wild-type, Km (NAD+) increased compared to wild-type Aeromonas hydrophila
N86A kcat moderately decreased compared to wild-type, Km decreased compared to wild-type Aeromonas hydrophila
S178A kcat decreased compared to wild-type, Km increased compared to wild-type Aeromonas hydrophila
Y77A kcat decreased compared to wild-type, Km increased compared to wild-type Aeromonas hydrophila
Y82A kcat decreased compared to wild-type, Km increased compared to wild-type Aeromonas hydrophila

Inhibitors

Inhibitors Comment Organism Structure
2-(4-[4-[(2,4-dimethoxyphenyl)amino]quinazolin-2-yl]piperazin-1-yl)ethanol
-
 Aeromonas hydrophila
N-(acridin-9-yl)-4-nitrobenzamide
-
 Aeromonas hydrophila
N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide
-
 Aeromonas hydrophila
suramin
-
 Aeromonas hydrophila
V8 protease
-
 Aeromonas hydrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 NAD+ mutant N86A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.005
-
 NAD+ mutant E213A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.006
-
 NAD+ wild-type, 25°C, pH not specified in the publication Aeromonas hydrophila
0.008
-
 NAD+ mutant Y77A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.011
-
 (ADP-D-ribosyl)n-actin mutant N86A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.012
-
 NAD+ mutant Y82A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.014
-
 NAD+ mutant E215A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.018
-
 NAD+ mutant S178A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.02
-
 (ADP-D-ribosyl)n-actin mutant E213A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.023
-
 (ADP-D-ribosyl)n-actin mutant E215A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.024
-
 (ADP-D-ribosyl)n-actin wild-type, 25°C, pH not specified in the publication Aeromonas hydrophila
0.033
-
 (ADP-D-ribosyl)n-actin mutant S178A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.035
-
 (ADP-D-ribosyl)n-actin mutant Y82A, 25°C, pH not specified in the publication Aeromonas hydrophila
0.037
-
 (ADP-D-ribosyl)n-actin mutant Y77A, 25°C, pH not specified in the publication Aeromonas hydrophila

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila Q49TP5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (ADP-D-ribosyl)n-actin VahC is shown to ADP-ribosylate Arg-177 of actin. VahC activity causes depolymerization of actin filaments, which induces caspase-mediated apoptosis in HeLa Tet-Off cells Aeromonas hydrophila nicotinamide + (ADP-D-ribosyl)n+1-actin
-
 ?

Synonyms

Synonyms Comment Organism
mART
-
 Aeromonas hydrophila
mono-ADP-ribosyltransferase
-
 Aeromonas hydrophila
VahC
-
 Aeromonas hydrophila
vsdc
-
 Aeromonas hydrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 NAD+ mutant E215A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
0.05
-
 (ADP-D-ribosyl)n-actin mutant E215A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
0.11
-
 NAD+ mutant E213A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
0.11
-
 (ADP-D-ribosyl)n-actin mutant E213A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
0.65
-
 NAD+ mutant Y82A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
0.65
-
 (ADP-D-ribosyl)n-actin mutant Y82A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
1.3
-
 NAD+ mutant Y77A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
1.3
-
 (ADP-D-ribosyl)n-actin mutant Y77A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
1.38
-
 NAD+ mutant S178, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
1.38
-
 (ADP-D-ribosyl)n-actin mutant S178A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
9.35
-
 NAD+ mutant N86A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
9.35
-
 (ADP-D-ribosyl)n-actin mutant N86A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
22
-
 NAD+ wild-type, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
22
-
 (ADP-D-ribosyl)n-actin wild-type, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
 suramin 25°C, pH not specified in the publication Aeromonas hydrophila
0.0041
-
 N-(acridin-9-yl)-4-nitrobenzamide 25°C, pH not specified in the publication Aeromonas hydrophila
0.0041
-
 N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide 25°C, pH not specified in the publication Aeromonas hydrophila
0.0075
-
 2-(4-[4-[(2,4-dimethoxyphenyl)amino]quinazolin-2-yl]piperazin-1-yl)ethanol 25°C, pH not specified in the publication Aeromonas hydrophila

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.02
-
 25°C, pH not specified in the publication Aeromonas hydrophila suramin
0.0858
-
 25°C, pH not specified in the publication Aeromonas hydrophila N-(acridin-9-yl)-4-nitrobenzamide
0.086
-
 25°C, pH not specified in the publication Aeromonas hydrophila N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide
0.157
-
 25°C, pH not specified in the publication Aeromonas hydrophila V8 protease