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Literature summary for 2.4.2.30 extracted from

  • Deng, Q.; Barbieri, J.T.
    Modulation of host cell endocytosis by the type III cytotoxin, Pseudomonas ExoS (2008), Traffic, 9, 1948-1957.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E381D site-directed mutagenesis, the mutation within the active site for the ADPr domain Pseudomonas aeruginosa
R146K site-directed mutagenesis, the mutation within the active site for the RhoGAP domain Pseudomonas aeruginosa
R146K/E379/381D inactive mutant, does not inhibit Pseudomonas aeruginosa internalization into ExoS-transfected HeLa cells in contrast to the wild-type enzyme Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa ExoS is a type III cytotoxin, that ADP-ribosylates Rab GTPases to inhibit host cell vesicle trafficking pathways by modulating HeLa host cell endocytosis, wild-type ExoS uncouples Rab5-early endosome antigen 1 interaction and inhibits fluid phase uptake, as well as Pseudomonas aeruginosa internalization, RhoGAP, but not ADPr, the ADPr domain is dispensable for anti-internalization function, but is required for inhibition of EGF-activated EGFR degradation in HeLa cells, mechanism, overview ?
-
?
additional information Pseudomonas aeruginosa PA103 ExoS is a type III cytotoxin, that ADP-ribosylates Rab GTPases to inhibit host cell vesicle trafficking pathways by modulating HeLa host cell endocytosis, wild-type ExoS uncouples Rab5-early endosome antigen 1 interaction and inhibits fluid phase uptake, as well as Pseudomonas aeruginosa internalization, RhoGAP, but not ADPr, the ADPr domain is dispensable for anti-internalization function, but is required for inhibition of EGF-activated EGFR degradation in HeLa cells, mechanism, overview ?
-
?
NAD+ + (ADP-D-ribosyl)n-acceptor Pseudomonas aeruginosa
-
nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
?
NAD+ + (ADP-D-ribosyl)n-acceptor Pseudomonas aeruginosa PA103
-
nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
?
NAD+ + Rab5 protein Pseudomonas aeruginosa Rab5 mediates entry of the EGFR into early endosomes nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+
-
?
NAD+ + Rab5 protein Pseudomonas aeruginosa PA103 Rab5 mediates entry of the EGFR into early endosomes nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+
-
?
NAD+ + Rab9 protein Pseudomonas aeruginosa
-
nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+
-
?
NAD+ + Rab9 protein Pseudomonas aeruginosa PA103
-
nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa PA103
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ExoS is a type III cytotoxin, that ADP-ribosylates Rab GTPases to inhibit host cell vesicle trafficking pathways by modulating HeLa host cell endocytosis, wild-type ExoS uncouples Rab5-early endosome antigen 1 interaction and inhibits fluid phase uptake, as well as Pseudomonas aeruginosa internalization, RhoGAP, but not ADPr, the ADPr domain is dispensable for anti-internalization function, but is required for inhibition of EGF-activated EGFR degradation in HeLa cells, mechanism, overview Pseudomonas aeruginosa ?
-
?
additional information ExoS is a bi-functional type III cytotoxin that possesses Rho GTPase activating protein and ADP-ribosyltransferase activities, no activity with Rab4 protein, overview Pseudomonas aeruginosa ?
-
?
additional information ExoS is a type III cytotoxin, that ADP-ribosylates Rab GTPases to inhibit host cell vesicle trafficking pathways by modulating HeLa host cell endocytosis, wild-type ExoS uncouples Rab5-early endosome antigen 1 interaction and inhibits fluid phase uptake, as well as Pseudomonas aeruginosa internalization, RhoGAP, but not ADPr, the ADPr domain is dispensable for anti-internalization function, but is required for inhibition of EGF-activated EGFR degradation in HeLa cells, mechanism, overview Pseudomonas aeruginosa PA103 ?
-
?
additional information ExoS is a bi-functional type III cytotoxin that possesses Rho GTPase activating protein and ADP-ribosyltransferase activities, no activity with Rab4 protein, overview Pseudomonas aeruginosa PA103 ?
-
?
NAD+ + (ADP-D-ribosyl)n-acceptor
-
Pseudomonas aeruginosa nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
?
NAD+ + (ADP-D-ribosyl)n-acceptor
-
Pseudomonas aeruginosa PA103 nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
?
NAD+ + Rab5 protein Rab5 mediates entry of the EGFR into early endosomes Pseudomonas aeruginosa nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+
-
?
NAD+ + Rab5 protein Rab5 mediates entry of the EGFR into early endosomes Pseudomonas aeruginosa PA103 nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+
-
?
NAD+ + Rab9 protein
-
Pseudomonas aeruginosa nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+
-
?
NAD+ + Rab9 protein
-
Pseudomonas aeruginosa PA103 nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+
-
?

Synonyms

Synonyms Comment Organism
ADP-ribosyltransferase
-
Pseudomonas aeruginosa
ADPr
-
Pseudomonas aeruginosa
exoenzyme S
-
Pseudomonas aeruginosa
ExoS
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas aeruginosa