Protein Variants | Comment | Organism |
---|---|---|
E381D | site-directed mutagenesis, the mutation within the active site for the ADPr domain | Pseudomonas aeruginosa |
R146K | site-directed mutagenesis, the mutation within the active site for the RhoGAP domain | Pseudomonas aeruginosa |
R146K/E379/381D | inactive mutant, does not inhibit Pseudomonas aeruginosa internalization into ExoS-transfected HeLa cells in contrast to the wild-type enzyme | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | ExoS is a type III cytotoxin, that ADP-ribosylates Rab GTPases to inhibit host cell vesicle trafficking pathways by modulating HeLa host cell endocytosis, wild-type ExoS uncouples Rab5-early endosome antigen 1 interaction and inhibits fluid phase uptake, as well as Pseudomonas aeruginosa internalization, RhoGAP, but not ADPr, the ADPr domain is dispensable for anti-internalization function, but is required for inhibition of EGF-activated EGFR degradation in HeLa cells, mechanism, overview | ? | - |
? | |
additional information | Pseudomonas aeruginosa PA103 | ExoS is a type III cytotoxin, that ADP-ribosylates Rab GTPases to inhibit host cell vesicle trafficking pathways by modulating HeLa host cell endocytosis, wild-type ExoS uncouples Rab5-early endosome antigen 1 interaction and inhibits fluid phase uptake, as well as Pseudomonas aeruginosa internalization, RhoGAP, but not ADPr, the ADPr domain is dispensable for anti-internalization function, but is required for inhibition of EGF-activated EGFR degradation in HeLa cells, mechanism, overview | ? | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | Pseudomonas aeruginosa | - |
nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | Pseudomonas aeruginosa PA103 | - |
nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | - |
? | |
NAD+ + Rab5 protein | Pseudomonas aeruginosa | Rab5 mediates entry of the EGFR into early endosomes | nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+ | - |
? | |
NAD+ + Rab5 protein | Pseudomonas aeruginosa PA103 | Rab5 mediates entry of the EGFR into early endosomes | nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+ | - |
? | |
NAD+ + Rab9 protein | Pseudomonas aeruginosa | - |
nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+ | - |
? | |
NAD+ + Rab9 protein | Pseudomonas aeruginosa PA103 | - |
nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa PA103 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ExoS is a type III cytotoxin, that ADP-ribosylates Rab GTPases to inhibit host cell vesicle trafficking pathways by modulating HeLa host cell endocytosis, wild-type ExoS uncouples Rab5-early endosome antigen 1 interaction and inhibits fluid phase uptake, as well as Pseudomonas aeruginosa internalization, RhoGAP, but not ADPr, the ADPr domain is dispensable for anti-internalization function, but is required for inhibition of EGF-activated EGFR degradation in HeLa cells, mechanism, overview | Pseudomonas aeruginosa | ? | - |
? | |
additional information | ExoS is a bi-functional type III cytotoxin that possesses Rho GTPase activating protein and ADP-ribosyltransferase activities, no activity with Rab4 protein, overview | Pseudomonas aeruginosa | ? | - |
? | |
additional information | ExoS is a type III cytotoxin, that ADP-ribosylates Rab GTPases to inhibit host cell vesicle trafficking pathways by modulating HeLa host cell endocytosis, wild-type ExoS uncouples Rab5-early endosome antigen 1 interaction and inhibits fluid phase uptake, as well as Pseudomonas aeruginosa internalization, RhoGAP, but not ADPr, the ADPr domain is dispensable for anti-internalization function, but is required for inhibition of EGF-activated EGFR degradation in HeLa cells, mechanism, overview | Pseudomonas aeruginosa PA103 | ? | - |
? | |
additional information | ExoS is a bi-functional type III cytotoxin that possesses Rho GTPase activating protein and ADP-ribosyltransferase activities, no activity with Rab4 protein, overview | Pseudomonas aeruginosa PA103 | ? | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | - |
Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | - |
Pseudomonas aeruginosa PA103 | nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | - |
? | |
NAD+ + Rab5 protein | Rab5 mediates entry of the EGFR into early endosomes | Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+ | - |
? | |
NAD+ + Rab5 protein | Rab5 mediates entry of the EGFR into early endosomes | Pseudomonas aeruginosa PA103 | nicotinamide + (ADP-D-ribosyl)-Rab5 protein + H+ | - |
? | |
NAD+ + Rab9 protein | - |
Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+ | - |
? | |
NAD+ + Rab9 protein | - |
Pseudomonas aeruginosa PA103 | nicotinamide + (ADP-D-ribosyl)-Rab9 protein + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribosyltransferase | - |
Pseudomonas aeruginosa |
ADPr | - |
Pseudomonas aeruginosa |
exoenzyme S | - |
Pseudomonas aeruginosa |
ExoS | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |