Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a soluble, cytosolic His function protein, rHisExoS | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
DELTAN222 | carboxyl-terminal 222 amino acids of exoenzyme S, catalyzes the ADP-ribosylation of soybean trypsin inhibitor at a rate sixfold greater than rHisExoS. Relative to rHisExoS, DN222 has a similar affinity for NAD, a threefold greater affinity for soybean trypsin inhibitor, and a four- to eight-fold greater turnover number for the ADP-ribosylation of soybean trypsin inhibitor. DN222 does not chromatograph as an aggregate, which shows that the amino-terminal 99 amino acids of exoenzyme S are responsible for the aggregation phenotype | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor | recombinant full-length enzyme | Pseudomonas aeruginosa | |
0.037 | - |
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor | recombinant DELTAN222 | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant cytosolic His function protein, rHisExoS | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (ADP-D-ribosyl)n-soybean-trypsin-inhibitor | - |
Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)n+1-soybean-trypsin-inhibitor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exoenzyme S | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor | recombinant DELTAN222 | Pseudomonas aeruginosa | |
0.008 | - |
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor | recombinant full-length enzyme | Pseudomonas aeruginosa |