Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.30 extracted from

  • Knight, D.A.; Finck-Barbancon, V.; Kulich, S.M.; Barbieri, J.T.
    Functional domains of Pseudomonas aeruginosa exoenzyme S (1995), Infect. Immun., 63, 3182-3186.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a soluble, cytosolic His function protein, rHisExoS Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
DELTAN222 carboxyl-terminal 222 amino acids of exoenzyme S, catalyzes the ADP-ribosylation of soybean trypsin inhibitor at a rate sixfold greater than rHisExoS. Relative to rHisExoS, DN222 has a similar affinity for NAD, a threefold greater affinity for soybean trypsin inhibitor, and a four- to eight-fold greater turnover number for the ADP-ribosylation of soybean trypsin inhibitor. DN222 does not chromatograph as an aggregate, which shows that the amino-terminal 99 amino acids of exoenzyme S are responsible for the aggregation phenotype Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor recombinant full-length enzyme Pseudomonas aeruginosa
0.037
-
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor recombinant DELTAN222 Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant cytosolic His function protein, rHisExoS Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (ADP-D-ribosyl)n-soybean-trypsin-inhibitor
-
Pseudomonas aeruginosa nicotinamide + (ADP-D-ribosyl)n+1-soybean-trypsin-inhibitor
-
?

Synonyms

Synonyms Comment Organism
exoenzyme S
-
Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0031
-
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor recombinant DELTAN222 Pseudomonas aeruginosa
0.008
-
(ADP-D-ribosyl)n-soybean-trypsin-inhibitor recombinant full-length enzyme Pseudomonas aeruginosa