Crystallization (Comment) | Organism |
---|---|
purified enzyme in complex with 2,2-anhydrouridine, X-ray diffraction structure determination and analysis at 1.34 A resolution, molecular replacement method using the structure of VchUPh in complex with 6-methyluracil (PDB ID 4K6O) as the starting model, from which all ligands, including enzyme-bound water molecules, are removed. The inhibitor molecules are located in all six active sites of the hexameric UPh molecule | Vibrio cholerae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-anhydrouridine | high-resolution three-dimensional structure of uridine phosphorylase from in complex with the competitive inhibitor determined by X-ray diffraction (PDB ID 6RCA), detailed binding structure analysis, overview. The binding of the inhibitor at the active site of the protein is energetically more favorable than the binding of the native substrate uridine. The inhibitor molecules are located in all six active sites of the hexameric UPh molecule. Effect of 2,2'-anhydrouridine binding on the conformation of structurally functional elements of VchUPh | Vibrio cholerae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + phosphate | Vibrio cholerae | - |
uracil + alpha-D-ribose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + phosphate | - |
Vibrio cholerae | uracil + alpha-D-ribose 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
VchUPh | - |
Vibrio cholerae |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure modeling, molecular dynamics simulation, overview. Calculation of the protein-ligand binding free energy | Vibrio cholerae |