Literature summary for 2.4.2.3 extracted from

da Silva Neto, A.M.; Torini de Souza, J.R.; Romanello, L.; Cassago, A.; Serrao, V.H.; DeMarco, R.; Brandao-Neto, J.; Garratt, R.C.; Pereira, H.D.
Analysis of two Schistosoma mansoni uridine phosphorylases isoforms suggests the emergence of a protein with a non-canonical function (2016), Biochimie, 125, 12-22.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Codon Plus cells	Schistosoma mansoni

Crystallization (Commentary)

Crystallization (Comment)	Organism
free isoform UPa and in complex with thymidine, uracil, thymine or 5-fluorouracil, sitting drop vapor diffusion method, using 100 mM trisodium citrate pH5.6, 16% (w/v) PEG4000 and 17.5% (v/v) isopropanol or 100 mM bis-Tris pH 5.5, 25% (w/v) PEG3350 and 200 mM ammonium sulfate	Schistosoma mansoni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.031	-	uridine	pH and temperature not specified in the publication	Schistosoma mansoni
0.0489	-	thymidine	pH and temperature not specified in the publication	Schistosoma mansoni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uridine + phosphate	Schistosoma mansoni	-	uracil + alpha-D-ribose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Schistosoma mansoni	G4VGH9	-	-

Purification (Commentary)

Purification (Comment)	Organism
Co-NTA agarose column chromatography	Schistosoma mansoni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thymidine + phosphate	-	Schistosoma mansoni	thymine + 2-deoxy-alpha-D-ribose 1-phosphate	-	?
uridine + phosphate	-	Schistosoma mansoni	uracil + alpha-D-ribose 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
uPA	isoform	Schistosoma mansoni
UPb	inactive isoform	Schistosoma mansoni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.41	-	thymidine	pH and temperature not specified in the publication	Schistosoma mansoni
35.9	-	uridine	pH and temperature not specified in the publication	Schistosoma mansoni

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
8.38	-	thymidine	pH and temperature not specified in the publication	Schistosoma mansoni
1158	-	uridine	pH and temperature not specified in the publication	Schistosoma mansoni

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Release 2023.1 (January 2023)