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Literature summary for 2.4.2.3 extracted from
- Purification and characterization of uridine and thymidine phosphorylase from Lactobacillus casei (1990), Biochim. Biophys. Acta, 1040, 287-293.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-O-methyl-alpha-D-glucopyranose | - |
Lacticaseibacillus casei |  |
| p-chloromercuribenzoate | - |
Lacticaseibacillus casei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Lacticaseibacillus casei | |
| 4 | - |
5-methyluridine | - |
Lacticaseibacillus casei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
4 * 20000, SDS-PAGE in presence of 2-mercaptoethanol | Lacticaseibacillus casei |
| 80000 | - |
non-denaturing PAGE | Lacticaseibacillus casei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lacticaseibacillus casei |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| uridine + phosphate = uracil + alpha-D-ribose 1-phosphate | sequential rather than ping-pong mechanism, addition of substrate is random | Lacticaseibacillus casei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2810 | - |
- |
Lacticaseibacillus casei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, 90% loss of activity after 3-4 days | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methyluridine + phosphate | - |
Lacticaseibacillus casei | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
| additional information | no substrate: cytidine | Lacticaseibacillus casei | ? | - |
? | |
| additional information | no substrates: 2-deoxyuridine, adenosine | Lacticaseibacillus casei | ? | - |
? | |
| thymidine + phosphate | - |
Lacticaseibacillus casei | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| uracil arabinoside + phosphate | - |
Lacticaseibacillus casei | uracil + arabinose-1-phosphate | - |
? | |
| uridine + phosphate | - |
Lacticaseibacillus casei | uracil + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 20000, SDS-PAGE in presence of 2-mercaptoethanol | Lacticaseibacillus casei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Lacticaseibacillus casei |
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Release 2023.1 (January 2023)
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