Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanosarcina acetivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of recombinant and native enzyme | Methanosarcina acetivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration : 10 mM | Methanosarcina acetivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT | Methanosarcina acetivorans |
54000 | - |
2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT | Methanosarcina acetivorans |
150000 | - |
gel filtration | Methanosarcina acetivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | Q8THU2 and Q8TUE6 | Q8THU2: Q8TUE6: | - |
Methanosarcina acetivorans DSM 2834 | Q8THU2 and Q8TUE6 | Q8THU2: Q8TUE6: | - |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina acetivorans |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT | Methanosarcina acetivorans |
Synonyms | Comment | Organism |
---|---|---|
ArcTGT | - |
Methanosarcina acetivorans |
tRNA-guanine transglycosylase | - |
Methanosarcina acetivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina acetivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Methanosarcina acetivorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 50% of maximal activity, pH 9.0: about 50% oif maximal activity | Methanosarcina acetivorans |
General Information | Comment | Organism |
---|---|---|
physiological function | in the first step of archaeosine biosynthesis, archaeosine tRNAguanine transglycosylase catalyzes the base exchange reaction from guanine to 7-cyano-7-deazaguanine | Methanosarcina acetivorans |