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Literature summary for 2.4.2.29 extracted from
- Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans (2013), Protein Expr. Purif., 88, 13-19.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Methanosarcina acetivorans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters of recombinant and native enzyme | Methanosarcina acetivorans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal concentration : 10 mM | Methanosarcina acetivorans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT | Methanosarcina acetivorans |
| 54000 | - |
2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT | Methanosarcina acetivorans |
| 150000 | - |
gel filtration | Methanosarcina acetivorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina acetivorans | Q8THU2 and Q8TUE6 | Q8THU2: Q8TUE6: | - |
| Methanosarcina acetivorans DSM 2834 | Q8THU2 and Q8TUE6 | Q8THU2: Q8TUE6: | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina acetivorans |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 2 * 19000 + 2 * 54000, archaeosine tRNAguanine transglycosylase is classified into full-size or split types. Although the full-size type forms a homodimeric structure, the split type forms a heterotetrameric structure, consisting of two kinds of peptide. Interaction between the two subunits may contribute to the conformational stability of split ArcTGT | Methanosarcina acetivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ArcTGT | - |
Methanosarcina acetivorans |
| tRNA-guanine transglycosylase | - |
Methanosarcina acetivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina acetivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Methanosarcina acetivorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7.0: about 50% of maximal activity, pH 9.0: about 50% oif maximal activity | Methanosarcina acetivorans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | in the first step of archaeosine biosynthesis, archaeosine tRNAguanine transglycosylase catalyzes the base exchange reaction from guanine to 7-cyano-7-deazaguanine | Methanosarcina acetivorans |
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Release 2023.1 (January 2023)
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