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Literature summary for 2.4.2.28 extracted from
- Transition-state structure of human 5-methylthioadenosine phosphorylase (2006), J. Am. Chem. Soc., 128, 14691-14696.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the N-terminally His6-tagged enzyme in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic isotope effects measured on the arsenolysis of 5'-methylthioadenosine catalyzed by the enzyme, the enzyme has a dissociative SN1 transition state with its cationic center at the anomeric carbon and insignificant bond order to the leaving group, the transition state has cationic character at the anomeric carbon and is anionic at the 3'-OH oxygen with an anionic leaving group, late SN1 transition state with significant participation of the phosphate nucleophile, overview | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-methyl-5'-thioadenosine + phosphate | Homo sapiens | - |
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | transition state structure, computational modeling of the transition state, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-methyl-5'-thioadenosine + arsenate | arsenolysis | Homo sapiens | adenine + S-methyl-5-thio-alpha-D-ribose 1-arsenate | - |
? | |
| S-methyl-5'-thioadenosine + phosphate | - |
Homo sapiens | adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? | |
| S-methyl-5'-thioadenosine + phosphate | phosphorolysis | Homo sapiens | adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5-methylthioadenosine phosphorylase | - |
Homo sapiens |
| MTAP | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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