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Literature summary for 2.4.2.28 extracted from

  • Cacciapuoti, G.; Bertoldo, C.; Brio, A.; Zappia, V.; Porcelli, M.
    Purification and characterization of 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus. Substrate specificity and primary structure analysis (2003), Extremophiles, 7, 159-168.
    View publication on PubMed
Search for more literature for 2.4.2.28

General Stability

General Stability Organism
dithiothreitol 0.8 M, reduction of thermostability Pyrococcus furiosus
enzyme is extremely stable to proteolytic cleavage Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 0.8 M, reduction of thermostability Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.109
-
 adenosine
-
 Pyrococcus furiosus
0.147
-
 5'-methylthioadenosine
-
 Pyrococcus furiosus
0.916
-
 guanosine
-
 Pyrococcus furiosus
0.963
-
 Inosine
-
 Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 6 * 30000, SDS-PAGE Pyrococcus furiosus
180000
-
 gel filtration Pyrococcus furiosus

Organic Solvent Stability

Organic Solvent Comment Organism
1-propanol at 50%: 88% remaining activity after 60 min at 50°C, 58% remaining activity after 60 min at 70°C, 22% remaining activity after 60 min at 90°C Pyrococcus furiosus
acetonitrile at 50%: stable at 90°C for 30 min, loss of 62% activity after 60 min Pyrococcus furiosus
dimethylformamide at 50%: 48% remaining activity after 60 min at 50°C, 5% remaining activity after 60 min at 70°C, no activity at 90°C Pyrococcus furiosus
Ethanol at 50%: 90% remaining activity after 60 min at 50°C, 65% remaining activity after 60 min at 70°C, 30% remaining activity after 60 min at 90°C Pyrococcus furiosus
Methanol at 50%: stable at 90°C for 30 min, loss of 77% activity after 60 min Pyrococcus furiosus
tetrahydrofuran at 50%: 77% remaining activity after 60 min at 50°C, 50% remaining activity after 60 min at 70°C, 3% remaining activity after 60 min at 90°C Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.35
-
 purified enzyme Pyrococcus furiosus

Storage Stability

Storage Stability Organism
20°C, Tris-HCl 10 mM, pH 7.4, purified enzyme, stable for at least 1 year Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + phosphate
-
 Pyrococcus furiosus adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
adenosine + phosphate
-
 Pyrococcus furiosus adenine + D-ribose 1-phosphate
-
 ?
guanosine + phosphate
-
 Pyrococcus furiosus guanine + D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Pyrococcus furiosus hypoxanthine + D-ribose 1-phosphate
-
 ?
additional information 6-amino purine nucleosides are the preferred substrates, substrate specificity Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 30000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Pyrococcus furiosus
125
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 160
-
 Pyrococcus furiosus
86 95 2 different activation energy-dependent processes occur below and above 100°C Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 5 h, 98% remaining activity Pyrococcus furiosus
130
-
 half-life: 43 min Pyrococcus furiosus
137
-
 melting temperature Pyrococcus furiosus
139
-
 melting temperature in presence of 100 mM phosphate Pyrococcus furiosus
140
-
 half-life: 13 min Pyrococcus furiosus
145
-
 half-life: 5 min Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.31
-
 guanosine
-
 Pyrococcus furiosus
9.38
-
 Inosine
-
 Pyrococcus furiosus
22.8
-
 adenosine
-
 Pyrococcus furiosus
24.5
-
 5'-methylthioadenosine
-
 Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Pyrococcus furiosus