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Literature summary for 2.4.2.28 extracted from

  • Zappia, V.; Oliva, A.; Cacciapuoti, G.; Galletti, P.; Mignucci, G.; Carteni-Farina, M.
    Substrate specificity of 5'-methylthioadenosine phosphorylase from human prostate (1978), Biochem. J., 175, 1043-1050.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol required Homo sapiens
dithiothreitol other thiols are less effective Homo sapiens
SH-group reducing agents requirement Homo sapiens

General Stability

General Stability Organism
Stable to freeze-thawing Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5'-dimethylthioadenosine sulfonium salt, noncompetitive Homo sapiens
5'-methylthiotubercidin competitive Homo sapiens
iodoacetamide reversal by dithiothreitol Homo sapiens
iodoacetate
-
Homo sapiens
p-chloromercuribenzoic acid strong, partially reversed by dithiothreitol Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
5'-methylthioadenosine
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Oxidation Stability

Oxidation Stability Organism
O2 partially inactivates Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate 3 binding sites between substrate and enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate gland
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.131
-
purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-ethylthioadenosine + phosphate 60% of the activity with 5'-methylthioadenosine Homo sapiens adenine + 5-ethylthio-D-ribose 1-phosphate
-
?
5'-methylselenoadenosine + phosphate 95% of the activity with 5'-methylthioadenosine Homo sapiens adenine + 5-methylseleno-D-ribose 1-phosphate
-
?
5'-methylthioadenosine + phosphate completely dependent on phosphate Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
?
5'-methylthioinosine + phosphate 8.9% of the reaction with 5'-methylthioadenosine Homo sapiens hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
?
additional information substrate specificity Homo sapiens ?
-
?
additional information replacement of the 6-amino group by an OH-group and of N-7 by a methinic radical results in almost complete loss of activity Homo sapiens ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
rapid inactivation Homo sapiens