Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | required | Homo sapiens | |
dithiothreitol | other thiols are less effective | Homo sapiens | |
SH-group reducing agents | requirement | Homo sapiens |
General Stability | Organism |
---|---|
Stable to freeze-thawing | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-dimethylthioadenosine | sulfonium salt, noncompetitive | Homo sapiens | |
5'-methylthiotubercidin | competitive | Homo sapiens | |
iodoacetamide | reversal by dithiothreitol | Homo sapiens | |
iodoacetate | - |
Homo sapiens | |
p-chloromercuribenzoic acid | strong, partially reversed by dithiothreitol | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
5'-methylthioadenosine | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
O2 partially inactivates | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | 3 binding sites between substrate and enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
prostate gland | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.131 | - |
purified enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-ethylthioadenosine + phosphate | 60% of the activity with 5'-methylthioadenosine | Homo sapiens | adenine + 5-ethylthio-D-ribose 1-phosphate | - |
? | |
5'-methylselenoadenosine + phosphate | 95% of the activity with 5'-methylthioadenosine | Homo sapiens | adenine + 5-methylseleno-D-ribose 1-phosphate | - |
? | |
5'-methylthioadenosine + phosphate | completely dependent on phosphate | Homo sapiens | adenine + 5-methylthio-D-ribose 1-phosphate | - |
? | |
5'-methylthioinosine + phosphate | 8.9% of the reaction with 5'-methylthioadenosine | Homo sapiens | hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate | - |
? | |
additional information | substrate specificity | Homo sapiens | ? | - |
? | |
additional information | replacement of the 6-amino group by an OH-group and of N-7 by a methinic radical results in almost complete loss of activity | Homo sapiens | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
rapid inactivation | Homo sapiens |