General Stability | Organism |
---|---|
addition of streptidine is important for stabilization of enzyme activity during purification, purified transferase is also stable in absence of streptidine | Streptomyces griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTDP | - |
Streptomyces griseus | |
dTTP | - |
Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | as effective as Mg2+ | Streptomyces griseus | |
Mg2+ | Mn2+ or Mg2+ required, maximal activity with 10 mM Mg2+ | Streptomyces griseus | |
Mn2+ | Mn2+ or Mg2+ required, maximal activity with 3 mM Mn2+ | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol | Streptomyces griseus |
63000 | - |
gel filtration | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-L-dihydrostreptose + streptidine 6-phosphate | Streptomyces griseus | biosynthesis of streptomycin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sephadex G-200, chromatography on DEAE-cellulose, Ultrogel AcA44 column, DEAE-Sepharose column | Streptomyces griseus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces griseus |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl, pH 7.8 at 0°C, containing 5 mM mercaptoethanol, 1 mM EDTA, 2.5 mM streptidine, 10% v/v glycerol, stable for at least 4 months | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-L-dihydrostreptose + streptidine 6-phosphate | - |
Streptomyces griseus | dTDP + O-alpha-L-dihydrostreptosyl-1,4-streptidine 6-phosphate | - |
? | |
dTDP-L-dihydrostreptose + streptidine 6-phosphate | biosynthesis of streptomycin | Streptomyces griseus | ? | - |
? | |
additional information | not: streptidine, 2-deoxystreptamine, 4-deoxystreptamine | Streptomyces griseus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol | Streptomyces griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
assay at | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no distinct pH-optimum observed in Tris-HCl buffer between pH 7.3 and 9 | Streptomyces griseus |
8.2 | - |
glycine/NaOH buffer | Streptomyces griseus |