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Literature summary for 2.4.2.27 extracted from

  • Kniep, B.; Grisebach, H.
    Biosynthesis of streptomycin. Purification and properties of a dTDP-L-dihydrostreptose:streptidine-6-phosphate dihydrostreptosyltransferase from Streptomyces griseus (1980), Eur. J. Biochem., 105, 139-144.
    View publication on PubMed

General Stability

General Stability Organism
addition of streptidine is important for stabilization of enzyme activity during purification, purified transferase is also stable in absence of streptidine Streptomyces griseus

Inhibitors

Inhibitors Comment Organism Structure
dTDP
-
Streptomyces griseus
dTTP
-
Streptomyces griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ as effective as Mg2+ Streptomyces griseus
Mg2+ Mn2+ or Mg2+ required, maximal activity with 10 mM Mg2+ Streptomyces griseus
Mn2+ Mn2+ or Mg2+ required, maximal activity with 3 mM Mn2+ Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol Streptomyces griseus
63000
-
gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-L-dihydrostreptose + streptidine 6-phosphate Streptomyces griseus biosynthesis of streptomycin ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-200, chromatography on DEAE-cellulose, Ultrogel AcA44 column, DEAE-Sepharose column Streptomyces griseus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptomyces griseus

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl, pH 7.8 at 0°C, containing 5 mM mercaptoethanol, 1 mM EDTA, 2.5 mM streptidine, 10% v/v glycerol, stable for at least 4 months Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-L-dihydrostreptose + streptidine 6-phosphate
-
Streptomyces griseus dTDP + O-alpha-L-dihydrostreptosyl-1,4-streptidine 6-phosphate
-
?
dTDP-L-dihydrostreptose + streptidine 6-phosphate biosynthesis of streptomycin Streptomyces griseus ?
-
?
additional information not: streptidine, 2-deoxystreptamine, 4-deoxystreptamine Streptomyces griseus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
-
-
assay at Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
no distinct pH-optimum observed in Tris-HCl buffer between pH 7.3 and 9 Streptomyces griseus
8.2
-
glycine/NaOH buffer Streptomyces griseus