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Literature summary for 2.4.2.26 extracted from

  • G๖tting, C.; Kuhn, J.; Zahn, R.; Brinkmann, T.; Kleesiek, K.
    Molecular cloning and expression of human UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase and its first isoform XT-II (2000), J. Mol. Biol., 304, 517-528.
    View publication on PubMed
Search for more literature for 2.4.2.26

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis of isoforms XT-I and XT-II, chromosome mapping Rattus norvegicus
DNA sequence determination and analysis, chromosome mapping, functional expression of isoforms XT-I and XT-II in CHO-K1 cells, no activity when XT-II is expressed fused to the aminoterminal peptide tag, 8% of the recombinant activity is located in the cytosol and membranes, 92% recombinant activity is secreted into the medium Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
heparin
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular in cell culture supernatant Homo sapiens
-
-
extracellular isoform XT-I Homo sapiens
-
-
membrane isoform XT-II, type II transmembrane protein Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
 x * 91000, isoform XT-I, amino acid sequence determination Homo sapiens
97000
-
 x * 97000, isoform XT-II, amino acid sequence determination Homo sapiens
120000
-
 gel filtration, amino acid sequence determination Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 2 isoforms XT-I and XT-II
-
Rattus norvegicus
-
 2 isoforms XT-I and XT-II
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 3 potential N-glycosylation sites, isoforms XT-I and XT-II Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
wild-type, and recombinant from CHO-K1 cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-xylose + [protein]-L-serine = UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine isoform XT-I contains the DxD motif at position 183, which has shown to be essential for binding of nucleotide sugars in glycosyltransferases Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
brain low content of isoform XT-II, and very low content of isoform XT-I Homo sapiens
-
choriocarcinoma cell
-
 Homo sapiens
-
heart
-
 Rattus norvegicus
-
heart isoform XT-II, low content Homo sapiens
-
JAR cell choriocarcinoma cell line Homo sapiens
-
kidney
-
 Rattus norvegicus
-
kidney isoforms XT-I and XT-II Homo sapiens
-
liver
-
 Rattus norvegicus
-
liver isoform XT-II Homo sapiens
-
lung
-
 Rattus norvegicus
-
lung isoform XT-II, and low content of isoform XT-I Homo sapiens
-
pancreas isoforms XT-I and XT-II Homo sapiens
-
placenta isoform XT-I and XT-II Homo sapiens
-
skeletal muscle isoform XT-II, low content Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-xylose + [protein]-L-serine transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxyl group of an acceptor protein substrate Homo sapiens UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?
UDP-alpha-D-xylose + [protein]-L-serine transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxyl group of an acceptor protein substrate Rattus norvegicus UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?

Subunits

Subunits Comment Organism
? x * 91000, isoform XT-I, amino acid sequence determination Homo sapiens
? x * 97000, isoform XT-II, amino acid sequence determination Homo sapiens
monomer
-
 Rattus norvegicus
monomer 1 * 120000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
XT
-
 Rattus norvegicus
XT 2 isoforms XT-I and XT-II Homo sapiens