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Literature summary for 2.4.2.22 extracted from

  • Anderson, B.W.; Hao, A.; Satyshur, K.A.; Keck, J.L.; Wang, J.D.
    Molecular mechanism of regulation of the purine salvage enzyme XPRT by the alarmones pppGpp, ppGpp, and pGpp (2020), J. Mol. Biol., 432, 4108-4126 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
pGpp competitive inhibitor Bacillus subtilis
ppGpp competitive inhibitor Bacillus subtilis
pppGpp competitive inhibitor Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
5-phospho-alpha-D-ribose 1-diphosphate at pH 7.6 and 25°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-phospho-alpha-D-ribose 1-diphosphate + xanthine Bacillus subtilis
-
XMP + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + xanthine Bacillus subtilis 168
-
XMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P42085
-
-
Bacillus subtilis 168 P42085
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA spin column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-phospho-alpha-D-ribose 1-diphosphate + xanthine
-
Bacillus subtilis XMP + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + xanthine
-
Bacillus subtilis 168 XMP + diphosphate
-
?

Subunits

Subunits Comment Organism
homodimer
-
Bacillus subtilis

Synonyms

Synonyms Comment Organism
XPRT
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57.3
-
melting temperature Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0025
-
pppGpp at pH 7.6 and 25°C Bacillus subtilis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00096
-
at pH 7.6 and 25°C Bacillus subtilis pGpp
0.0033
-
at pH 7.6 and 25°C Bacillus subtilis ppGpp
0.0564
-
at pH 7.6 and 25°C Bacillus subtilis pppGpp