Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pGpp | competitive inhibitor | Bacillus subtilis | |
ppGpp | competitive inhibitor | Bacillus subtilis | |
pppGpp | competitive inhibitor | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.6 and 25°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM used in assay conditions | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate + xanthine | Bacillus subtilis | - |
XMP + diphosphate | - |
? | |
5-phospho-alpha-D-ribose 1-diphosphate + xanthine | Bacillus subtilis 168 | - |
XMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P42085 | - |
- |
Bacillus subtilis 168 | P42085 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA spin column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate + xanthine | - |
Bacillus subtilis | XMP + diphosphate | - |
? | |
5-phospho-alpha-D-ribose 1-diphosphate + xanthine | - |
Bacillus subtilis 168 | XMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
XPRT | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57.3 | - |
melting temperature | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
pppGpp | at pH 7.6 and 25°C | Bacillus subtilis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00096 | - |
at pH 7.6 and 25°C | Bacillus subtilis | pGpp | |
0.0033 | - |
at pH 7.6 and 25°C | Bacillus subtilis | ppGpp | |
0.0564 | - |
at pH 7.6 and 25°C | Bacillus subtilis | pppGpp |