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Literature summary for 2.4.2.22 extracted from

  • Miller, R.L.; Adamczyk, D.L.; Fyfe, J.A.; Elion, G.B.
    Xanthine phosphoribosyltransferase from Streptococcus faecalis. Properties and specificity (1974), Arch. Biochem. Biophys., 165, 349-358.
    View publication on PubMed

General Stability

General Stability Organism
increase in potassium phosphate buffer concentration from 1-50 mM increases stability Enterococcus faecalis
stabilizing compounds are: 5-phospho-alpha-D-ribose 1-diphosphate, XMP, 6-oxo-substituted purine nucleotides Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
1-Methylxanthine
-
Enterococcus faecalis
2,4-dihydroxy-5,6-tetramethylenepyrimidine
-
Enterococcus faecalis
2,4-Dihydroxypteridine
-
Enterococcus faecalis
2,4-dihydroxypyrido[2,3-d]pyrimidine
-
Enterococcus faecalis
2,4-dihydroxypyrido[3,2-d]pyrimidine
-
Enterococcus faecalis
2,4-dihydroxypyrido[3,4-d]pyrimidine
-
Enterococcus faecalis
2,4-dihydroxypyrrolo[2,3-d]pyrimidine
-
Enterococcus faecalis
2,6-dithioxanthine
-
Enterococcus faecalis
2-hydroxy-6-methylthiopurine
-
Enterococcus faecalis
2-Hydroxypurine
-
Enterococcus faecalis
2-mercaptopurine
-
Enterococcus faecalis
2-methylhypoxanthine
-
Enterococcus faecalis
2-methylthio-6-hydroxypurine
-
Enterococcus faecalis
2-Thioxanthine
-
Enterococcus faecalis
3-Methylxanthine
-
Enterococcus faecalis
4,6-dihydroxypyrazolo[3,4-d]-pyrimidine
-
Enterococcus faecalis
4-aminoimidazole-5-carboxamide
-
Enterococcus faecalis
4-hydroxypyrazolo[3,4-d]pyrimidine
-
Enterococcus faecalis
4-mercapto-6-hydroxypyrazolo[3,4-d]pyrimidine
-
Enterococcus faecalis
4-mercaptopyrazolo[3,4-d]pyrimidine
-
Enterococcus faecalis
5,7-dihydroxy(1,2,5)thiadiazolo[3,4-d]pyrimidine
-
Enterococcus faecalis
5,7-dihydroxy-v-triazolo[4,5-d]pyrimidine
-
Enterococcus faecalis
5,7-dihydroxypyrazolo[4,3-d]pyrimidine
-
Enterococcus faecalis
5-acetamidouracil
-
Enterococcus faecalis
5-Aminouracil
-
Enterococcus faecalis
5-anilinouracil
-
Enterococcus faecalis
5-formamidouracil
-
Enterococcus faecalis
5-phospho-alpha-D-ribose 1-diphosphate
-
Enterococcus faecalis
6-Mercaptopurine
-
Enterococcus faecalis
6-Thioxanthine
-
Enterococcus faecalis
7-methylxanthine
-
Enterococcus faecalis
8-mercaptoxanthine
-
Enterococcus faecalis
8-methylxanthine
-
Enterococcus faecalis
9-methylxanthine
-
Enterococcus faecalis
adenine
-
Enterococcus faecalis
ATP 1 mM, 11% inhibition Enterococcus faecalis
CDP 1 mM, 10% inhibition Enterococcus faecalis
CTP 1 mM, 27% inhibition Enterococcus faecalis
GDP 1 mM, 93 inhibition Enterococcus faecalis
GMP 1 mM, 89% inhibition Enterococcus faecalis
GTP 1 mM, 91% inhibition Enterococcus faecalis
guanine
-
Enterococcus faecalis
hypoxanthine
-
Enterococcus faecalis
isoguanine
-
Enterococcus faecalis
purine
-
Enterococcus faecalis
TDP 1 mM, 18% inhibition Enterococcus faecalis
TTP 1 mM, 13% inhibition Enterococcus faecalis
UDP 1 mM, 15% inhibition Enterococcus faecalis
Uracil
-
Enterococcus faecalis
uric acid
-
Enterococcus faecalis
UTP 1 mM, 17% inhibition Enterococcus faecalis
xanthine
-
Enterococcus faecalis
XDP 1 mM, 89% inhibition Enterococcus faecalis
XMP 1 mM, 92% inhibition Enterococcus faecalis
XTP 1 mM, 92% inhibition Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
5,7-dihydroxy-v-triazolo[4,5-d]pyrimidine
-
Enterococcus faecalis
0.02
-
xanthine
-
Enterococcus faecalis
0.053
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.65
-
-
Enterococcus faecalis

Storage Stability

Storage Stability Organism
-70°C, stable for 6 months in intacts cells Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-phospho-alpha-D-ribose 1-diphosphate + 4,6-dihydroxypyrazolo[3,4-d]pyrimidine at 0.064% of the activity with xanthine Enterococcus faecalis ?
-
?
5-phospho-alpha-D-ribose 1-diphosphate + 5,7-dihydroxy-v-triazolo[4,5-d]pyrimidine at 16.8% of the activity with xanthine Enterococcus faecalis ?
-
?
5-phospho-alpha-D-ribose 1-diphosphate + adenine at 0.25% of the activity with xanthine Enterococcus faecalis AMP + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine at 0.19% of the activity with xanthine Enterococcus faecalis IMP + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + xanthine
-
Enterococcus faecalis 9-(5-phospho-beta-D-ribosyl)xanthine + diphosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
pH 5.6-10, stable for 30 min Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.8
-
Enterococcus faecalis

pH Range

pH Minimum pH Maximum Comment Organism
6.9 9.1 80% of maximal activity at pH 6.9 and 9.1 Enterococcus faecalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.6 10 stable Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0022
-
5,7-dihydroxypyrazolo[4,3-d]pyrimidine
-
Enterococcus faecalis
0.0041
-
2,4-dihydroxypyrido[3,2-d]pyrimidine
-
Enterococcus faecalis
0.021
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Enterococcus faecalis
0.024
-
xanthine
-
Enterococcus faecalis
0.024
-
2,4-Dihydroxypteridine
-
Enterococcus faecalis
0.032
-
5,7-dihydroxy(1,2,5)thiadiazolo[3,4-d]pyrimidine
-
Enterococcus faecalis
0.11
-
5,7-dihydroxy-v-triazolo[4,5-d]pyrimidine
-
Enterococcus faecalis
0.15
-
2,6-dithioxanthine
-
Enterococcus faecalis
0.17
-
2-hydroxy-6-methylthiopurine
-
Enterococcus faecalis
0.2
-
4-mercapto-6-hydroxypyrazolo[3,4-d]pyrimidine
-
Enterococcus faecalis
0.31
-
4,6-dihydroxypyrazolo[3,4-d]-pyrimidine
-
Enterococcus faecalis
0.34
-
6-Thioxanthine
-
Enterococcus faecalis
0.38
-
2-Thioxanthine
-
Enterococcus faecalis
0.39
-
8-mercaptoxanthine
-
Enterococcus faecalis
0.62
-
3-Methylxanthine
-
Enterococcus faecalis