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Literature summary for 2.4.2.20 extracted from
- 3-Ribosylpurines. I.Synthesis of (3-ribosyluric acid) 5phosphate and (3-ribosylxanthine) 5phosphate by a pyrimidine ribonucleotide pyrophosphorylase (1964), J. Biol. Chem., 239, 2580-2586.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-ribosylxanthine 5'-phosphate | xanthine and uracil pyrophosphorylase | Bos taurus |  |
| UMP | xanthine and uracil pyrophosphorylase | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required at 2 mM or higher for maximal activity | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified 5400fold, ammonium sulfate fractionation, chromatography on DEAE-cellulose, treatment with Alumina CY Gel, calcium phosphate gel adsorption and elution and purification with hydroxylapatite | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.497 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme also synthesizes a number of pyrimidine ribonucleotides from the corresponding base, e.g. uracil, orotic acid, thymine, 6-azathymine, 6-azauracil, 5-fluorouracil or 5-iodouracil and diphosphorylribose phosphate | Bos taurus | ? | - |
? | |
| uric acid + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Bos taurus | 3-ribosyluric acid 5'-phosphate + diphosphate | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Bos taurus | 3-ribosylxanthine 5'-phosphate + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 6.0, 40 min, 12-22% loss of activity | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
for uric acid reaction | Bos taurus |
| 7.6 | 8.6 | for xanthine reaction | Bos taurus |
| 8 | 9.2 | for orotic acid reaction | Bos taurus |
| 9.5 | - |
for uracil reaction | Bos taurus |
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Release 2023.1 (January 2023)
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