Any feedback?
Literature summary for 2.4.2.2 extracted from
- A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides (2017), Biotechnol. Lett., 39, 1903-1910 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons | Siminovitchia fordii |
| gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons | Brevibacillus borstelensis |
| gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons | Aneurinibacillus migulanus |
| gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons | Lacticaseibacillus paracasei |
| gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons | Serratia marcescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Siminovitchia fordii | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Brevibacillus borstelensis | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Aneurinibacillus migulanus | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Lacticaseibacillus paracasei | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Serratia marcescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymidine + phosphate | Brevibacillus borstelensis | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? |  |
| thymidine + phosphate | Brevibacillus borstelensis LK01 | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | Brevibacillus borstelensis | - |
uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | Brevibacillus borstelensis LK01 | - |
uracil + alpha-D-ribose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aneurinibacillus migulanus | A0A0D1XZI7 | - |
- |
| Aneurinibacillus migulanus U603 | A0A0D1XZI7 | - |
- |
| Brevibacillus borstelensis | A0A1S5VH74 | - |
- |
| Brevibacillus borstelensis LK01 | A0A1S5VH74 | - |
- |
| Lacticaseibacillus paracasei | A0A0E2M090 | - |
- |
| Serratia marcescens | A0A080UUN2 | - |
- |
| Serratia marcescens MH6 | A0A080UUN2 | - |
- |
| Siminovitchia fordii | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.4 | - |
purified recombinant enzyme, pH 8.0, 50°C, substrate uridine | Aneurinibacillus migulanus |
| 37.5 | - |
purified recombinant enzyme, pH 8.0, 50°C, substrate uridine | Lacticaseibacillus paracasei |
| 43.3 | - |
purified recombinant enzyme, pH 8.5, 50°C, substrate uridine | Serratia marcescens |
| 55.8 | - |
purified recombinant enzyme, pH 8.0, 50°C, substrate uridine | Siminovitchia fordii |
| 98.3 | - |
purified recombinnat enzyme, pH 8.5, 50°C, substrate uridine | Brevibacillus borstelensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyuridine + phosphate | - |
Brevibacillus borstelensis | uracil + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| 5-fluoro-2'-deoxyuridine + phosphate | - |
Brevibacillus borstelensis | 5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| 5-fluoro-2'-deoxyuridine + phosphate | - |
Brevibacillus borstelensis LK01 | 5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| 5-methyluridine + phosphate | - |
Brevibacillus borstelensis | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
| 5-methyluridine + phosphate | - |
Brevibacillus borstelensis LK01 | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
| additional information | under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine | Brevibacillus borstelensis | ? | - |
- |
|
| additional information | under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine | Brevibacillus borstelensis LK01 | ? | - |
- |
|
| thymidine + phosphate | - |
Brevibacillus borstelensis | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| thymidine + phosphate | - |
Brevibacillus borstelensis LK01 | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Siminovitchia fordii | uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Brevibacillus borstelensis | uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Aneurinibacillus migulanus | uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Lacticaseibacillus paracasei | uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Serratia marcescens | uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Serratia marcescens MH6 | uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Brevibacillus borstelensis LK01 | uracil + alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Aneurinibacillus migulanus U603 | uracil + alpha-D-ribose 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AmPyNP | - |
Aneurinibacillus migulanus |
| AmPyNP | - |
Serratia marcescens |
| BbPyNP | - |
Brevibacillus borstelensis |
| BfPyNP | - |
Siminovitchia fordii |
| deoA | - |
Aneurinibacillus migulanus |
| deoA | - |
Lacticaseibacillus paracasei |
| deoA | - |
Serratia marcescens |
| LpPyNP | - |
Lacticaseibacillus paracasei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Siminovitchia fordii |
| 50 | - |
assay at | Brevibacillus borstelensis |
| 50 | - |
assay at | Aneurinibacillus migulanus |
| 50 | - |
assay at | Lacticaseibacillus paracasei |
| 50 | - |
assay at | Serratia marcescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | activity range, profile overview, recombinant enzyme | Brevibacillus borstelensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme, pH 8.5, 1 h, 90% activity remaining | Brevibacillus borstelensis |
| 45 | - |
purified recombinant enzyme, pH 8.5, 1 h, 80% activity remaining | Brevibacillus borstelensis |
| 50 | - |
purified recombinant enzyme, pH 8.5, 1 h, 43.7% activity remaining | Lacticaseibacillus paracasei |
| 50 | - |
purified recombinant enzyme, pH 8.5, 1 h, 49.6% activity remaining | Siminovitchia fordii |
| 50 | - |
purified recombinant enzyme, pH 8.5, 1 h, 52.1% activity remaining | Serratia marcescens |
| 50 | - |
purified recombinant enzyme, pH 8.5, 1 h, 65.6% activity remaining | Aneurinibacillus migulanus |
| 50 | - |
purified recombinant enzyme, pH 8.5, 1 h, 75% activity remaining | Brevibacillus borstelensis |
| 55 | - |
purified recombinant enzyme, pH 8.5, 1 h, 70% activity remaining | Brevibacillus borstelensis |
| 60 | - |
purified recombinant enzyme, pH 8.5, 1 h, 10% activity remaining | Brevibacillus borstelensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Brevibacillus borstelensis |
| 8.5 | - |
assay at | Siminovitchia fordii |
| 8.5 | - |
assay at | Aneurinibacillus migulanus |
| 8.5 | - |
assay at | Lacticaseibacillus paracasei |
| 8.5 | - |
assay at | Serratia marcescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10.5 | activity range, profile overview, recombinant enzyme | Brevibacillus borstelensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.8 | purified enzyme, stable at | Brevibacillus borstelensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
