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Literature summary for 2.4.2.2 extracted from
- Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus (1969), J. Biol. Chem., 244, 3691-3697.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
uridine | - |
Geobacillus stearothermophilus |  |
| 0.38 | - |
thymidine | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
sedimentation velocity analysis | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-bromodeoxyuridine + phosphate | 95% of the activity with uridine | Geobacillus stearothermophilus | 5-bromouracil + deoxyribose 1-phosphate | - |
? | |
| 5-bromouridine + phosphate | 95% of the activity with uridine | Geobacillus stearothermophilus | 5-bromouracil + alpha-D-ribose 1-phosphate | - |
? | |
| cytidine + phosphate | 8% of the activity with uridine | Geobacillus stearothermophilus | cytosine + alpha-D-ribose 1-phosphate | - |
? | |
| deoxyuridine + phosphate | 179% of the activity with uridine, by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed | Geobacillus stearothermophilus | uracil + 2-deoxy-D-ribose 1-phosphate | - |
r | |
| additional information | no activity with deoxycytidine | Geobacillus stearothermophilus | ? | - |
? | |
| thymidine + phosphate | 139% of the activity with uridine | Geobacillus stearothermophilus | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed | Geobacillus stearothermophilus | uracil + alpha-D-ribose 1-phosphate | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
60 min, stable | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
uridine, thymidine | Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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