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Literature summary for 2.4.2.2 extracted from

  • Scocca, J.J.
    Purification and substrate specificity of pyrimidine nucleoside phosphorylase from Haemophilus influenzae (1971), J. Biol. Chem., 246, 6606-6610.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
uridine above 15 mM, competitive inhibition of cleavage of 5-bromodeoxyuridine Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
5-Bromouridine
-
Haemophilus influenzae
0.07
-
5-methyluridine
-
Haemophilus influenzae
0.1
-
5-bromodeoxyuridine
-
Haemophilus influenzae
0.11
-
thymidine
-
Haemophilus influenzae
0.13
-
deoxyuridine
-
Haemophilus influenzae
0.24
-
uridine
-
Haemophilus influenzae
0.38
-
phosphate reaction with uridine Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haemophilus influenzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Haemophilus influenzae

Storage Stability

Storage Stability Organism
-20°C, in presence of phosphate, glycerol and dithiothreitol, stable for 6 months Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromodeoxyuridine + phosphate
-
Haemophilus influenzae 5-bromouracil + deoxyribose 1-phosphate
-
?
5-bromouridine + phosphate
-
Haemophilus influenzae 5-bromouracil + alpha-D-ribose 1-phosphate
-
?
5-methyluridine + phosphate
-
Haemophilus influenzae 5-methyluracil + alpha-D-ribose 1-phosphate
-
?
cytidine + phosphate no activity Haemophilus influenzae cytosine + alpha-D-ribose 1-phosphate
-
?
deoxyuridine + phosphate
-
Haemophilus influenzae uracil + 2-deoxy-D-ribose 1-phosphate
-
?
thymidine + phosphate
-
Haemophilus influenzae thymine + 2-deoxy-D-ribose 1-phosphate
-
?
uridine + phosphate
-
Haemophilus influenzae uracil + alpha-D-ribose 1-phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
deoxynucleoside substrates Haemophilus influenzae
7.4
-
ribonucleosides and uracil arabinoside Haemophilus influenzae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
uridine
-
Haemophilus influenzae