Literature summary for 2.4.2.19 extracted from

Booth, W.T.; Morris, T.L.; Mysona, D.P.; Shah, M.J.; Taylor, L.K.; Karlin, T.W.; Clary, K.; Majorek, K.A.; Offermann, L.R.; Chruszcz, M.
Streptococcus pyogenes quinolinate-salvage pathway-structural and functional studies of quinolinate phosphoribosyl transferase and NH3 -dependent NAD+ synthetase (2017), FEBS J., 284, 2425-2441 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptococcus pyogenes serotype M4

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging or sitting drop vapor diffusion method, using 0.48 M ammonium sulfate, 25% (w/v) PEG 6000 and 0.1 M bis-Tris (pH 6.5)	Streptococcus pyogenes serotype M4

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity, 6 mM used in assay conditions	Streptococcus pyogenes serotype M4

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
204000	-	gel filtration	Streptococcus pyogenes serotype M4
212300	-	dynamic light scattering	Streptococcus pyogenes serotype M4

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphoribosyl diphosphate + quinolinic acid	Streptococcus pyogenes serotype M4	-	nicotinate mononucleotide + diphosphate + CO2	-	r
phosphoribosyl diphosphate + quinolinic acid	Streptococcus pyogenes serotype M4 MGAS 10750	-	nicotinate mononucleotide + diphosphate + CO2	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pyogenes serotype M4	Q1J647	-	-
Streptococcus pyogenes serotype M4 MGAS 10750	Q1J647	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration	Streptococcus pyogenes serotype M4

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoribosyl diphosphate + quinolinic acid	-	Streptococcus pyogenes serotype M4	nicotinate mononucleotide + diphosphate + CO2	-	r
phosphoribosyl diphosphate + quinolinic acid	-	Streptococcus pyogenes serotype M4 MGAS 10750	nicotinate mononucleotide + diphosphate + CO2	-	r

Subunits

Subunits	Comment	Organism
hexamer	-	Streptococcus pyogenes serotype M4

Synonyms

Synonyms	Comment	Organism
quinolinate phosphoribosyl transferase	-	Streptococcus pyogenes serotype M4
quinolinate phosphoribosyltansferase	-	Streptococcus pyogenes serotype M4
spNadC	-	Streptococcus pyogenes serotype M4

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Release 2023.1 (January 2023)