Literature summary for 2.4.2.18 extracted from

Perveen, S.; Rashid, N.; Tang, X.F.; Imanaka, T.; Papageorgiou, A.C.
Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Thermococcus kodakarensis shows maximum activity with zinc and forms a unique dimeric structure (2017), FEBS Open Bio, 7, 1217-1230 .

Search for more literature for 2.4.2.18

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells	Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
free enzyme and in the presence of Zn2+, hanging drop vapor diffusion method, using 0.2 M sodium acetate (pH 7.5), 20% (w/v) PEG 3350	Thermococcus kodakarensis

Inhibitors

Inhibitors	Comment	Organism	Structure
anthranilate	substrate inhibition. Concentrations above 0.004 mM result in reduced enzymatic activity	Thermococcus kodakarensis
Co2+	-	Thermococcus kodakarensis
Mg2+	concentrations higher than 0.2 mM Mg2+ inhibit the enzyme activity (about 85% residual activity at 0.25 mM, about 70% residual activity at 0.5 mM, about 60% residual activity at 1.0 mM)	Thermococcus kodakarensis
Mn2+	-	Thermococcus kodakarensis
Zn2+	concentrations higher than 0.1 mM Zn2+ inhibit the enzyme activity (about 80% residual activity at 0.3 mM, about 65% residual activity at 0.5 mM, about 20% residual activity at 1.0 mM)	Thermococcus kodakarensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0022	-	anthranilate	at pH 8.5 and 55°C	Thermococcus kodakarensis
0.25	-	5-phospho-alpha-D-ribose 1-diphosphate	at pH 8.5 and 55°C	Thermococcus kodakarensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Thermococcus kodakarensis
Cu2+	slightly activates	Thermococcus kodakarensis
Mg2+	activates at 0.2 mM	Thermococcus kodakarensis
additional information	not influenced by Ni2+	Thermococcus kodakarensis
Zn2+	the enzyme shows highest activity with 0.1 mM Zn2+. The enzyme contains a total of 6 Zn2+ ions in the dimer	Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	gel filtration	Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate	Thermococcus kodakarensis	-	N-(5-phospho-D-ribosyl)-anthranilate + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	Q9YGB4	-	-

Purification (Commentary)

Purification (Comment)	Organism
anion exchange QFF column chromatography, Resource Q column chromatography and Superdex 75 gel filtration	Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1580	-	at pH 8.5 and 55°C	Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate	-	Thermococcus kodakarensis	N-(5-phospho-D-ribosyl)-anthranilate + diphosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 36000, SDS-PAGE	Thermococcus kodakarensis
homodimer	2 * 34347, calculated from amino acid sequence	Thermococcus kodakarensis

Synonyms

Synonyms	Comment	Organism
TrpD	-	Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	9	-	Thermococcus kodakarensis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermococcus kodakarensis	calculated from amino acid sequence	-	4.9

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Release 2023.1 (January 2023)