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Literature summary for 2.4.2.18 extracted from
- Purification and characterization of yeast anthranilate phosphoribosyltransferase (1989), Eur. J. Biochem., 180, 33-40.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
anthranilate | - |
Saccharomyces cerevisiae |  |
| 0.0224 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Saccharomyces cerevisiae |
| 83000 | - |
sedimentation equilibrium | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharomyces cerevisiae | enzyme of tryptophan biosynthesis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 95% pure | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.58 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| addition of 10% glycerol at -70°C and then dropping the solution into liquid N2, can be thawed and frozen several times without loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | enzyme of tryptophan biosynthesis | Saccharomyces cerevisiae | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
loss of 10% activity in 24 h | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.9 | - |
anthranilate | cosubstrate: 5-phospho-alpha-D-ribose 1-diphosphate | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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