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Literature summary for 2.4.2.17 extracted from
- Guarding the gateway to histidine biosynthesis in plants Medicago truncatula ATP-phosphoribosyltransferase in relaxed and tense states (2018), Biochem. J., 475, 2681-2697 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 gold cells | Medicago truncatula |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| native and SeMet-labeled enzyme, hanging drop vapor diffusion method, using 2.53 M NaCl, 100 mM Bis-Tris propane pH 6.3, and 10% (v/v) glycerol | Medicago truncatula |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | competitive inhibition | Medicago truncatula |  |
| L-histidine | complete inhibition at 0.1 mM | Medicago truncatula | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.182 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.0 and 24°C | Medicago truncatula | |
| 0.47 | - |
ATP | at pH 8.0 and 24°C | Medicago truncatula | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Medicago truncatula | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Medicago truncatula | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Medicago truncatula | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Medicago truncatula | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago truncatula | G7JFL4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap resin column chromatography and Superdex S200 gel filtration | Medicago truncatula |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Medicago truncatula | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Medicago truncatula | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Medicago truncatula | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | x-ray crystallography | Medicago truncatula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-PRT1 | - |
Medicago truncatula |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.57 | - |
ATP | at pH 8.0 and 24°C | Medicago truncatula | |
| 0.57 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.0 and 24°C | Medicago truncatula | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0061 | - |
in the presence of 2 mM AMP, at pH 8.0 and 24°C | Medicago truncatula | L-histidine | |
| 0.0301 | - |
in the absence of AMP, at pH 8.0 and 24°C | Medicago truncatula | L-histidine | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
ATP | at pH 8.0 and 24°C | Medicago truncatula | |
| 3.1 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.0 and 24°C | Medicago truncatula | |
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Release 2023.1 (January 2023)
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