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Literature summary for 2.4.2.17 extracted from

  • Champagne, K.S.; Piscitelli, E.; Francklyn, C.S.
    Substrate recognition by the hetero-octameric ATP phosphoribosyltransferase from Lactococcus lactis (2006), Biochemistry, 45, 14933-14943.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.17

Protein Variants

Protein Variants Comment Organism
D155A slight increase in kcat, 3.9fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 1.7fold increase in Km-value for ATP Lactococcus lactis
K50A 2.3fold increase in kcat, 51fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 1.8fold increase in Km-value for ATP Lactococcus lactis
K8A 2.5fold decrease in kcat, 4.9fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 3.1fold increase in Km-value for ATP Lactococcus lactis
S140A mutant is unstable, kinetic parameters can not be determined Lactococcus lactis
T159A 2.2fold decrease in kcat, 280fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 2.5fold decrease in Km-value for ATP Lactococcus lactis
T162A 3.2fold decrease in kcat, 49fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 2.6fold decrease in Km-value for ATP Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive Lactococcus lactis
histidine noncompetitive Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0184
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, wild-type enzyme Lactococcus lactis
0.0712
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme D155A Lactococcus lactis
0.09
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K8A Lactococcus lactis
0.901
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T162A Lactococcus lactis
0.94
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K50A Lactococcus lactis
1.05
-
 ATP 25°C, pH 8, mutant enzyme T162A Lactococcus lactis
1.07
-
 ATP 25°C, pH 8, mutant enzyme T159A Lactococcus lactis
2.7
-
 ATP 25°C, pH 8, wild-type enzyme Lactococcus lactis
4.7
-
 ATP 25°C, pH 8, mutant enzyme D155A Lactococcus lactis
5
-
 ATP 25°C, pH 8, mutant enzyme K50A Lactococcus lactis
5.15
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T159A Lactococcus lactis
8.45
-
 ATP 25°C, pH 8, mutant enzyme K8A Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Lactococcus lactis first reaction of histidine biosynthesis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate first reaction of histidine biosynthesis Lactococcus lactis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
octamer four HisGS catalytic subunits related to periplasmic binding proteins and four HisZ regulatory subunits that resemble histidyl-tRNA synthetases Lactococcus lactis

Synonyms

Synonyms Comment Organism
ATP-PRT
-
 Lactococcus lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K50A Lactococcus lactis
0.84
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T162A Lactococcus lactis
1.07
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K8A Lactococcus lactis
1.2
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T159A Lactococcus lactis
2.7
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, wild-type enzyme Lactococcus lactis
2.83
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme D155A Lactococcus lactis
6.16
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K50A Lactococcus lactis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0811
-
 histidine 25°C, pH 8 Lactococcus lactis
1.44
-
 AMP 25°C, pH 8 Lactococcus lactis