Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.16 extracted from

  • Laster, L.; Blair, A.
    An intestinal phosphorylase for uric ribonucleoside (1963), J. Biol. Chem., 238, 3348-3357.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-Ribosyluracil 1 mM, about 85% inhibition Canis lupus familiaris
2-thio-6-oxopurine 1 mM, 73% inhibition Canis lupus familiaris
2-Thiouracil 1 mM, 96% inhibition Canis lupus familiaris
4-amino-5-imidazolecarboxamide 1 mM, about 15% inhibition Canis lupus familiaris
5-methyluracil 1 mM, about 90% inhibition Canis lupus familiaris
5-methyluridine 1 mM, about 95% inhibition Canis lupus familiaris
Colchicine 1 mM, 43% inhibition, competitive Canis lupus familiaris
guanosine 1 mM, about 20% inhibition Canis lupus familiaris
Inosine 1 mM, about 25% inhibition Canis lupus familiaris
p-chloromercuribenzoate reversed by cysteine Canis lupus familiaris
Phenylbutazone 1 mM, 30% inhibition, competitive Canis lupus familiaris
Uracil 1 mM, about 85% inhibition Canis lupus familiaris
xanthine 1 mM, 35% inhibition Canis lupus familiaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uric acid ribonucleoside + phosphate Cavia porcellus
-
urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + phosphate Rattus norvegicus
-
urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + phosphate Pigeon
-
urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + phosphate Canis lupus familiaris
-
urate + D-ribose 1-phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Cavia porcellus
-
-
-
Pigeon
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Cavia porcellus
-
heart
-
Rattus norvegicus
-
heart
-
Pigeon
-
kidney
-
Cavia porcellus
-
kidney
-
Rattus norvegicus
-
kidney
-
Pigeon
-
kidney
-
Canis lupus familiaris
-
liver
-
Rattus norvegicus
-
liver
-
Pigeon
-
skeletal muscle
-
Rattus norvegicus
-
small intestine mucosa
-
Rattus norvegicus
-
small intestine mucosa
-
Pigeon
-
small intestine mucosa
-
Canis lupus familiaris
-
spleen low activity Rattus norvegicus
-
testis
-
Rattus norvegicus
-
testis
-
Pigeon
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
-
Canis lupus familiaris

Storage Stability

Storage Stability Organism
-10°C, partially purified enzyme is stable for at least 2 months Canis lupus familiaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uric acid ribonucleoside + arsenate
-
Cavia porcellus urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + arsenate
-
Rattus norvegicus urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + arsenate
-
Pigeon urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + arsenate reverse reaction not demonstrated, arsenate can replace phosphate at a lower reaction rate Canis lupus familiaris urate + D-ribose 1-phosphate
-
ir
uric acid ribonucleoside + phosphate
-
Cavia porcellus urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + phosphate
-
Rattus norvegicus urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + phosphate
-
Pigeon urate + D-ribose 1-phosphate
-
?
uric acid ribonucleoside + phosphate
-
Canis lupus familiaris urate + D-ribose 1-phosphate
-
ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 7.8, 5 min, 5% loss of activity, partially purified enzyme Canis lupus familiaris
60
-
pH 7.8, 5 min, 40% loss of activity, partially purified enzyme Canis lupus familiaris
70
-
pH 7.8, 5 min, 95% loss of activity, partially purified enzyme Canis lupus familiaris
80
-
pH 7.8, 5 min, 100% loss of activity, partially purified enzyme Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 reaction with phosphate or arsenate Canis lupus familiaris

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 60% of maximal activity, pH 9.5: 45% of maximal activity Canis lupus familiaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
0°C, 10 min, 30% loss of activity, partially purified enzyme Canis lupus familiaris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.23
-
Colchicine 37°C, pH 8.2 Canis lupus familiaris
1.76
-
Phenylbutazone 37°C, pH 8.2 Canis lupus familiaris