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Literature summary for 2.4.2.12 extracted from

  • Khan, J.A.; Tao, X.; Tong, L.
    Molecular basis for the inhibition of human NMPRTase, a novel target for anticancer agents (2006), Nat. Struct. Mol. Biol., 13, 582-588.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures at up to 2.1 A resolution of NMPRTase, alone and in complex with the reaction product nicotinamide mononucleotide or the inhibitor FK866. Crystals of the selenomethionyl free enzyme of human NMPRTase (F132M I151M double mutant) are grown with the sitting drop vapor diffusion method at 22°C. Crystals of wild-type human NMPRTase in complex with NMN are obtained at 22°C by sitting drop vapor diffusion Homo sapiens
crystal structures at up to 2.1 A resolution of NMPRTase, alone and in complex with the reaction product nicotinamide mononucleotide or the inhibitor FK866. Crystals of wild-type murine NMPRTase free enzyme are obtained by sitting drop vapor diffusion at 4°C Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
FK866 FK866 is bound in a tunnel at the interface of the NMPRTase dimer, the inhibitor can reduce cellular NAD+ levels and induce apoptosis in tumors Homo sapiens
FK866 FK866 is bound in a tunnel at the interface of the NMPRTase dimer, the inhibitor can reduce cellular NAD+ levels and induce apoptosis in tumors Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus NMPRTase is a crucial enzyme in the salvage pathway of NAD+ biosynthesis and has important functions in regulating NAD+ levels in cells undergoing substantial NAD+ turnover ?
-
?
additional information Homo sapiens NMPRTase is a crucial enzyme in the salvage pathway of NAD+ biosynthesis and has important functions in regulating NAD+ levels in cells undergoing substantial NAD+ turnover ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P43490
-
-
Mus musculus Q99KQ4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NMPRTase is a crucial enzyme in the salvage pathway of NAD+ biosynthesis and has important functions in regulating NAD+ levels in cells undergoing substantial NAD+ turnover Mus musculus ?
-
?
additional information NMPRTase is a crucial enzyme in the salvage pathway of NAD+ biosynthesis and has important functions in regulating NAD+ levels in cells undergoing substantial NAD+ turnover Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
NMPRTase
-
Mus musculus
NMPRTase
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Homo sapiens