Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JW3617(DE3) cells | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 100 mM ammonium sulfate, 30% (w/v) PEG 3350, at 4°C | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
orotidine 5'-phosphate + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8N0R1 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, HiTrap Q-Sepharose column chromatography, and S200 XK gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | orotidine 5'-phosphate + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
OPRTase | - |
Plasmodium falciparum |