Inhibitors | Comment | Organism | Structure |
---|---|---|---|
forodesine | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine | ? | - |
- |
|
additional information | Rattus norvegicus Wistar-Kyoto | in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00491 | - |
- |
Rattus norvegicus | P85973 | - |
- |
Rattus norvegicus Wistar-Kyoto | P85973 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | recombinant enzyme | Homo sapiens | - |
fibroblast | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
mesangial cell | - |
Rattus norvegicus | - |
vascular smooth muscle cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methyl-6-thioguanosine + phosphate | - |
Homo sapiens | 7-methyl-6-thioguanine + alpha-D-ribose 1-phosphate | - |
r | |
inosine + phosphate | complete conversion | Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
additional information | in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine | Rattus norvegicus | ? | - |
- |
|
additional information | the recombinant enzyme does not metabolize adenosine to adenine or N6-etheno-adenosine to N6-etheno-adenine | Homo sapiens | ? | - |
- |
|
additional information | in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine | Rattus norvegicus Wistar-Kyoto | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
monomer | the commercial recombinant enzyme is in monomeric form | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PNPase | - |
Homo sapiens |
PNPase | - |
Rattus norvegicus |
purine nucleoside phosphorylase | - |
Homo sapiens |
purine nucleoside phosphorylase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.4 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
additional information | N6-etheno-ATP and its downstream metabolites are devoid of pharmacological activity | Rattus norvegicus |
physiological function | PNP catalyzes a reversible phosphorolysis of the N-glycosidic bond in natural purine nucleosides, as well as inmany purine analogues, some of them displaying marked biological and pharmacological activity | Homo sapiens |
physiological function | PNP catalyzes a reversible phosphorolysis of the N-glycosidic bond in natural purine nucleosides, as well as inmany purine analogues, some of them displaying marked biological and pharmacological activity | Rattus norvegicus |