Any feedback?
Literature summary for 2.4.2.1 extracted from
- Thermophilic nucleoside phosphorylases their properties, characteristics and applications (2020), Biochim. Biophys. Acta, 1868, 140304 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme is useful in biocatalytic production of 2,6-diaminopurine 2'-deoxyriboside | Thermus thermophilus |
| synthesis | enzyme is useful in biocatalytic production of 2,6-diaminopurine riboside and 5-methyluridine | Aeropyrum pernix |
| synthesis | enzyme is useful in biocatalytic production of 2,6-diaminopurine riboside, 2,6-dichloropurine riboside, 6-chloro-2-fluoropurine riboside, 2-chloroadenine riboside, 2-fluoroadenine riboside, 2,6-diaminopurine 2'-deoxyriboside, 2,6-dichloropurine 2'-deoxyriboside, 6-chloro-2-fluoropurine 2'-deoxyriboside, 2-chloro-2'-deoxyadenosine (cladribine), and 2-fluoro-2'-deoxyadenosine, as well as of 2,6-diaminopurine arabinoside, 2,6-dichloropurine arabinoside, and 6-chloro-2-fluoropurine arabinoside | Parageobacillus thermoglucosidasius |
| synthesis | enzyme is useful in biocatalytic production of 2-chloro-2'-deoxyadenosine (Cladribine), and 2-fluoroadenine arabinoside | Geobacillus stearothermophilus |
| synthesis | enzyme is useful in biocatalytic production of 2-fluoro-2'-deoxyadenosine and 2'-deoxy-2'-fluoroadenosine | Deinococcus geothermalis |
| synthesis | enzyme is useful in biocatalytic production of 5-methyluridine | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + phosphate | Parageobacillus thermoglucosidasius | - |
adenine + alpha-D-ribose 1-phosphate | - |
r |  |
| adenosine + phosphate | Pyrococcus furiosus | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Deinococcus geothermalis | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Thermus thermophilus | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Aeropyrum pernix | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Geobacillus stearothermophilus | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Pyrococcus furiosus ATCC 43587 | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Thermus thermophilus DSM 7039 | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Pyrococcus furiosus Vc1 | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Deinococcus geothermalis DSM 11300 | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Deinococcus geothermalis AG-3a | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Parageobacillus thermoglucosidasius 11955 | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Pyrococcus furiosus JCM 8422 | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | Thermus thermophilus ATCC BAA-163 | - |
adenine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Parageobacillus thermoglucosidasius | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Pyrococcus furiosus | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Deinococcus geothermalis | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Thermus thermophilus | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Aeropyrum pernix | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Geobacillus stearothermophilus | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Pyrococcus furiosus ATCC 43587 | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Thermus thermophilus DSM 7039 | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Pyrococcus furiosus Vc1 | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Deinococcus geothermalis DSM 11300 | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Deinococcus geothermalis AG-3a | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Parageobacillus thermoglucosidasius 11955 | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Pyrococcus furiosus JCM 8422 | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | Thermus thermophilus ATCC BAA-163 | - |
hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | A0A401H9D3 | - |
- |
| Deinococcus geothermalis | - |
- |
- |
| Deinococcus geothermalis AG-3a | - |
- |
- |
| Deinococcus geothermalis DSM 11300 | - |
- |
- |
| Geobacillus stearothermophilus | P77834 | - |
- |
| Geobacillus stearothermophilus | P77835 | - |
- |
| Parageobacillus thermoglucosidasius | - |
i.e. Parageobacillus thermoglucosidasius | - |
| Parageobacillus thermoglucosidasius 11955 | - |
i.e. Parageobacillus thermoglucosidasius | - |
| Pyrococcus furiosus | Q8U2I1 | - |
- |
| Pyrococcus furiosus ATCC 43587 | Q8U2I1 | - |
- |
| Pyrococcus furiosus JCM 8422 | Q8U2I1 | - |
- |
| Pyrococcus furiosus Vc1 | Q8U2I1 | - |
- |
| Thermus thermophilus | Q72IR2 | - |
- |
| Thermus thermophilus | Q72L69 | - |
- |
| Thermus thermophilus ATCC BAA-163 | Q72IR2 | - |
- |
| Thermus thermophilus ATCC BAA-163 | Q72L69 | - |
- |
| Thermus thermophilus DSM 7039 | Q72IR2 | - |
- |
| Thermus thermophilus DSM 7039 | Q72L69 | - |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the enzyme is stable at 5%-20% DMSO | Parageobacillus thermoglucosidasius |
| the enzyme is stable at 5%-20% DMSO | Deinococcus geothermalis |
| the enzyme is stable at 50% DMF, 50% DMSO, and 50% 2-MeTHF | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + phosphate | - |
Parageobacillus thermoglucosidasius | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Pyrococcus furiosus | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Deinococcus geothermalis | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Thermus thermophilus | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Aeropyrum pernix | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Geobacillus stearothermophilus | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Pyrococcus furiosus ATCC 43587 | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Thermus thermophilus DSM 7039 | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Pyrococcus furiosus Vc1 | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Deinococcus geothermalis DSM 11300 | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Deinococcus geothermalis AG-3a | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Parageobacillus thermoglucosidasius 11955 | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Pyrococcus furiosus JCM 8422 | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| adenosine + phosphate | - |
Thermus thermophilus ATCC BAA-163 | adenine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Parageobacillus thermoglucosidasius | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Pyrococcus furiosus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Deinococcus geothermalis | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Thermus thermophilus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Aeropyrum pernix | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Geobacillus stearothermophilus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Pyrococcus furiosus ATCC 43587 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Thermus thermophilus DSM 7039 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Pyrococcus furiosus Vc1 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Deinococcus geothermalis DSM 11300 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Deinococcus geothermalis AG-3a | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Parageobacillus thermoglucosidasius 11955 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Pyrococcus furiosus JCM 8422 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Thermus thermophilus ATCC BAA-163 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Parageobacillus thermoglucosidasius | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Pyrococcus furiosus | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Deinococcus geothermalis | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Thermus thermophilus | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Aeropyrum pernix | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Geobacillus stearothermophilus | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Pyrococcus furiosus ATCC 43587 | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Thermus thermophilus DSM 7039 | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Pyrococcus furiosus Vc1 | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Deinococcus geothermalis DSM 11300 | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Deinococcus geothermalis AG-3a | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Parageobacillus thermoglucosidasius 11955 | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Pyrococcus furiosus JCM 8422 | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Thermus thermophilus ATCC BAA-163 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| apPNP | - |
Aeropyrum pernix |
| DeoD | - |
Geobacillus stearothermophilus |
| deoD_1 | gene name, UniProt | Parageobacillus thermoglucosidasius |
| DgPNP | - |
Deinococcus geothermalis |
| GsPNPI | - |
Geobacillus stearothermophilus |
| GsPNPII | - |
Geobacillus stearothermophilus |
| GtPNP | - |
Parageobacillus thermoglucosidasius |
| PNP | - |
Parageobacillus thermoglucosidasius |
| PNP | - |
Pyrococcus furiosus |
| PNP | - |
Deinococcus geothermalis |
| PNP | - |
Thermus thermophilus |
| PNP | - |
Aeropyrum pernix |
| PNP | - |
Geobacillus stearothermophilus |
| PNP 1 | - |
Geobacillus stearothermophilus |
| PNP 2 | - |
Geobacillus stearothermophilus |
| PNP I | - |
Geobacillus stearothermophilus |
| PNP II | - |
Geobacillus stearothermophilus |
| pNPI | - |
Thermus thermophilus |
| PNPII | - |
Thermus thermophilus |
| punA | - |
Geobacillus stearothermophilus |
| purine nucleoside phosphorylase 1 | - |
Geobacillus stearothermophilus |
| purine nucleoside phosphorylase 2 | - |
Parageobacillus thermoglucosidasius |
| purine nucleoside phosphorylase 2 | - |
Geobacillus stearothermophilus |
| purine nucleoside phosphorylase DeoD-type | UniProt | Geobacillus stearothermophilus |
| TTC0194 | locus name | Thermus thermophilus |
| TTC1070 | locus name | Thermus thermophilus |
| TtPNPI | - |
Thermus thermophilus |
| TtPNPII | - |
Thermus thermophilus |
| ylmD | - |
Parageobacillus thermoglucosidasius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Parageobacillus thermoglucosidasius |
| 70 | - |
- |
Deinococcus geothermalis |
| 70 | - |
- |
Geobacillus stearothermophilus |
| 80 | - |
- |
Geobacillus stearothermophilus |
| 99 | - |
above | Thermus thermophilus |
| 100 | - |
- |
Aeropyrum pernix |
| 120 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermophilic signature of the enzyme, overview | Parageobacillus thermoglucosidasius |
| additional information | - |
thermophilic signature of the enzyme, overview | Pyrococcus furiosus |
| additional information | - |
thermophilic signature of the enzyme, overview | Deinococcus geothermalis |
| additional information | - |
thermophilic signature of the enzyme, overview | Thermus thermophilus |
| additional information | - |
thermophilic signature of the enzyme, overview | Aeropyrum pernix |
| additional information | - |
thermophilic signature of the enzyme, overview | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life is over 8 h | Parageobacillus thermoglucosidasius |
| 60 | - |
half-life is 1.6 h | Parageobacillus thermoglucosidasius |
| 70 | - |
half-life is over 3 h | Geobacillus stearothermophilus |
| 70 | - |
half-life is over 8 h | Deinococcus geothermalis |
| 80 | - |
half-life is 132 h | Thermus thermophilus |
| 80 | - |
half-life is 15 min | Geobacillus stearothermophilus |
| 80 | - |
half-life is 232 h | Thermus thermophilus |
| 100 | - |
half-life is over 60 min | Aeropyrum pernix |
| 105 | - |
half-life is 69 min | Pyrococcus furiosus |
| 110 | - |
half-life is 12 min | Pyrococcus furiosus |
| 115 | - |
half-life is 5 min | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermus thermophilus |
| 5 | - |
- |
Aeropyrum pernix |
| 5 | 6 | - |
Thermus thermophilus |
| 7 | - |
- |
Parageobacillus thermoglucosidasius |
| 7 | - |
- |
Pyrococcus furiosus |
| 7 | - |
- |
Deinococcus geothermalis |
| 7 | 11 | - |
Geobacillus stearothermophilus |
| 7.5 | 11 | - |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Thermus thermophilus |
| 3 | 11 | - |
Aeropyrum pernix |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
