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Literature summary for 2.4.1.B84 extracted from

  • Okitsu, N.; Matsui, K.; Horikawa, M.; Sugahara, K.; Tanaka, Y.
    Identification and characterization of novel nemophila menziesii flavone glucosyltransferases that catalyze biosynthesis of flavone 7,4'-O -diglucoside, a key component of blue metalloanthocyanins (2018), Plant Cell Physiol., 59, 2075-2085 .
    View publication on PubMed
Search for more literature for 2.4.1.B84

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 apigenin pH 7.5, 30°C Nemophila menziesii

Organism

Organism UniProt Comment Textmining
Nemophila menziesii A0A292GFI2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Nemophila menziesii
-
leaf poor expression Nemophila menziesii
-
petal highly expressed during the early stages of petal development Nemophila menziesii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: apigenin 4'-O-glucoside, luteolin 4'-O-glucoside, apigenin 7-O-glucoside or luteolin 7-O-glucoside, genistein, daidzein or betanidin Nemophila menziesii ?
-
-
UDP-alpha-D-glucose + kaempferol 14.5% of the activity with apigenin Nemophila menziesii UDP + kaempferol 4'-O-glucoside
-
 ?
UDP-alpha-D-glucose + luteolin 52.6% of the activity with apigenin Nemophila menziesii UDP + luteolin 4'-O-glucoside
-
 ?
UDP-alpha-D-glucose + quercetin 62.4% of the activity with apigenin Nemophila menziesii UDP + quercetin 4'-O-glucoside
-
 ?
UDP-D-glucose + apigenin
-
 Nemophila menziesii UDP + apigenin 4'-O-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
F4'GT
-
 Nemophila menziesii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Nemophila menziesii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 apigenin pH 7.5, 30°C Nemophila menziesii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Nemophila menziesii

General Information

General Information Comment Organism
physiological function Nicotiana tabacum cells expressing both UDP-glucose:flavone 4'-O-glycosyltransferase and F4'G7GT synthesize apigenin 7,4'-O-diglucoside in presence of apigenin Nemophila menziesii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 apigenin pH 7.5, 30°C Nemophila menziesii