Literature summary for 2.4.1.B83 extracted from

Diretto, G.; Ahrazem, O.; Rubio-Moraga, A.; Fiore, A.; Sevi, F.; Argandona, J.; Gomez-Gomez, L.
UGT709G1 a novel uridine diphosphate glycosyltransferase involved in the biosynthesis of picrocrocin, the precursor of safranal in saffron (Crocus sativus) (2019), New Phytol., 224, 725-740 .

Search for more literature for 2.4.1.B83

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Crocus sativus

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling of structure. The predicted substrate tunnel is formed by the amino acid residues P16, G17, Q18, G19, H20, R45, F82, L89, F201, L290, H359, G361, F381, A382, and Q384	Crocus sativus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.064	-	2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene	pH 8.0, 30°C	Crocus sativus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Crocus sativus	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-D-glucose + 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene	Crocus sativus	-	UDP + picrocrocin	-	?

Organism

Organism	UniProt	Comment	Textmining
Crocus sativus	A0A343AXU3	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	no expression is detected in leaves, corms, or roots	Crocus sativus	-
stigma	expression of UGT709G1 occurs preferentially in the earlier developmental stages, with low transcripts levels also detected in fully developed stigmas	Crocus sativus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no substrates: crocetin, quercetin, kaempferol, dihydrokaempferol, rhamnetin, isorhamnetin, lutein, apigenin, myricetin, caffeic acid, coumaric acid, genistein, esculetin, trans-cinnamic acid, sinapic acid, indole acetic acid, and abscisic acid	Crocus sativus	?	-	-
UDP-D-glucose + 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene	-	Crocus sativus	UDP + picrocrocin	-	?

Subunits

Subunits	Comment	Organism
?	x * 57500, calculated from sequence	Crocus sativus

Synonyms

Synonyms	Comment	Organism
UGT709G1	-	Crocus sativus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0074	-	2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene	pH 8.0, 30°C	Crocus sativus

General Information

General Information	Comment	Organism
physiological function	coexpression of CCD2L and UGT709G1 in Nicothiana benthamiana leads to interception of carotenoid flux to cleave zeaxanthin and to produce picrocrocin	Crocus sativus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.11	-	2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene	pH 8.0, 30°C	Crocus sativus

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Release 2023.1 (January 2023)