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Literature summary for 2.4.1.B77 extracted from

  • Faucard, P.; Grimaud, F.; Lourdin, D.; Maigret, J.; Moulis, C.; Remaud-Simeon, M.; Putaux, J.; Potocki-Veronese, G.; Rolland-Sabate, A.
    Macromolecular structure and film properties of enzymatically-engineered high molar mass dextrans (2018), Carbohydr. Polym., 181, 337-344 .
    View publication on PubMed
Search for more literature for 2.4.1.B77

Application

Application Comment Organism
synthesis alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview Leuconostoc citreum

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Escherichia coli strain BL21 Leuconostoc citreum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (1->6)-alpha-D-glucan Leuconostoc citreum
-
 D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch
-
 ?
sucrose + (1->6)-alpha-D-glucan Leuconostoc citreum NRRL B-742
-
 D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch
-
 ?
sucrose + (1->6)-alpha-D-glucan Leuconostoc citreum NRRL B-1299
-
 D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch
-
 ?

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum
-
-
-
Leuconostoc citreum NRRL B-1299
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages Leuconostoc citreum ?
-
-
additional information alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages Leuconostoc citreum NRRL B-742 ?
-
-
additional information alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages Leuconostoc citreum NRRL B-1299 ?
-
-
sucrose + (1->6)-alpha-D-glucan
-
 Leuconostoc citreum D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch
-
 ?
sucrose + (1->6)-alpha-D-glucan
-
 Leuconostoc citreum NRRL B-742 D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch
-
 ?
sucrose + (1->6)-alpha-D-glucan
-
 Leuconostoc citreum NRRL B-1299 D-fructose + (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch
-
 ?

Synonyms

Synonyms Comment Organism
BRS-A
-
 Leuconostoc citreum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Leuconostoc citreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.75
-
 assay at Leuconostoc citreum

General Information

General Information Comment Organism
evolution a distinct subgroup of sucrose-active enzymes is included in the GH70 family, corresponding to enzymes that display no polymerase activity, but efficiently transfer the glucosyl residue from sucrose to linear alpha(1->6) dextrans through the specific formation of single alpha(1->2) or alpha(1->3) linked glucosyl units. They are named branching sucrases (BRSs) Leuconostoc citreum
additional information branching induces a glass transition (Tg) depression (-10/-20°C) for amylopectin vs amylose and (-5/-10°C) for phytoglycogen vs amylopectin, which can be interpreted in terms of internal plasticization Leuconostoc citreum