Any feedback?
Literature summary for 2.4.1.B62 extracted from
- Difference in mono-O-glucosylation of Ras subtype GTPases between toxin A and toxin B from Clostridioides difficile strain 10463 and lethal toxin from Clostridium sordellii strain 6018 (2018), Front. Microbiol., 9, 3078 .
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Clostridioides difficile |  |
| Mg2+ | required | Paeniclostridium sordellii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + a small GTPase | Clostridioides difficile | - |
UDP + D-glucosyl-[a small GTPase] | - |
? | |
| UDP-alpha-D-glucose + a small GTPase | Paeniclostridium sordellii | - |
UDP + D-glucosyl-[a small GTPase] | - |
? | |
| UDP-alpha-D-glucose + a small GTPase | Paeniclostridium sordellii 6018 | - |
UDP + D-glucosyl-[a small GTPase] | - |
? | |
| UDP-alpha-D-glucose + a small GTPase | Clostridioides difficile 10463 | - |
UDP + D-glucosyl-[a small GTPase] | - |
? | |
| UDP-alpha-D-glucose + human Ras-GTPase | Clostridioides difficile | activity of toxin A in human epithelial colorectal adenocarcinoma Caco-2 cells | UDP + D-glucosyl-[human Ras-GTPase] | - |
? | |
| UDP-alpha-D-glucose + human Ras-GTPase | Paeniclostridium sordellii | activity of toxin TcsL in human epithelial colorectal adenocarcinoma Caco-2 cells | UDP + D-glucosyl-[human Ras-GTPase] | - |
? | |
| UDP-alpha-D-glucose + human Ras-GTPase | Paeniclostridium sordellii 6018 | activity of toxin TcsL in human epithelial colorectal adenocarcinoma Caco-2 cells | UDP + D-glucosyl-[human Ras-GTPase] | - |
? | |
| UDP-alpha-D-glucose + human Ras-GTPase | Clostridioides difficile 10463 | activity of toxin A in human epithelial colorectal adenocarcinoma Caco-2 cells | UDP + D-glucosyl-[human Ras-GTPase] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridioides difficile | P16154 | - |
- |
| Clostridioides difficile | P18177 | - |
- |
| Clostridioides difficile 10463 | P16154 | - |
- |
| Clostridioides difficile 10463 | P18177 | - |
- |
| Paeniclostridium sordellii | Q46342 | i.e. Clostridium sordellii | - |
| Paeniclostridium sordellii 6018 | Q46342 | i.e. Clostridium sordellii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Clostridioides difficile toxin B is not active on human Ras-GTPase in human epithelial colorectal adenocarcinoma Caco-2 cells | Clostridioides difficile | ? | - |
- |
|
| additional information | Clostridioides difficile toxin B is not active on human Ras-GTPase in human epithelial colorectal adenocarcinoma Caco-2 cells | Clostridioides difficile 10463 | ? | - |
- |
|
| UDP-alpha-D-glucose + a small GTPase | - |
Clostridioides difficile | UDP + D-glucosyl-[a small GTPase] | - |
? | |
| UDP-alpha-D-glucose + a small GTPase | - |
Paeniclostridium sordellii | UDP + D-glucosyl-[a small GTPase] | - |
? | |
| UDP-alpha-D-glucose + a small GTPase | - |
Paeniclostridium sordellii 6018 | UDP + D-glucosyl-[a small GTPase] | - |
? | |
| UDP-alpha-D-glucose + a small GTPase | - |
Clostridioides difficile 10463 | UDP + D-glucosyl-[a small GTPase] | - |
? | |
| UDP-alpha-D-glucose + human Ras-GTPase | activity of toxin A in human epithelial colorectal adenocarcinoma Caco-2 cells | Clostridioides difficile | UDP + D-glucosyl-[human Ras-GTPase] | - |
? | |
| UDP-alpha-D-glucose + human Ras-GTPase | activity of toxin TcsL in human epithelial colorectal adenocarcinoma Caco-2 cells | Paeniclostridium sordellii | UDP + D-glucosyl-[human Ras-GTPase] | - |
? | |
| UDP-alpha-D-glucose + human Ras-GTPase | activity of toxin TcsL in human epithelial colorectal adenocarcinoma Caco-2 cells | Paeniclostridium sordellii 6018 | UDP + D-glucosyl-[human Ras-GTPase] | - |
? | |
| UDP-alpha-D-glucose + human Ras-GTPase | activity of toxin A in human epithelial colorectal adenocarcinoma Caco-2 cells | Clostridioides difficile 10463 | UDP + D-glucosyl-[human Ras-GTPase] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Clostridium sordellii lethal toxin | - |
Paeniclostridium sordellii |
| glucosyltransferase TcdA | - |
Clostridioides difficile |
| glucosyltransferase TcdB | - |
Clostridioides difficile |
| TcdA | - |
Clostridioides difficile |
| TcdB | - |
Clostridioides difficile |
| TcsL | - |
Paeniclostridium sordellii |
| toxin | - |
Paeniclostridium sordellii |
| toxin A | - |
Clostridioides difficile |
| toxin B | - |
Clostridioides difficile |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridioides difficile |
| 37 | - |
assay at | Paeniclostridium sordellii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Clostridioides difficile |
| 7.4 | - |
assay at | Paeniclostridium sordellii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Clostridioides difficile toxin A (TcdA) and Toxin B (TcdB) trigger inflammasome activation with caspase-1 activation in cultured cells, which in turn induce the release of IL-6, IFN-g, and IL-8. Release of these proinflammatory responses is positively regulated by Ras-GTPases, which leads to the hypothesis that Ras glucosylation by glucosylating toxins results in (at least) reduced proinflammatory responses. Quantitative evaluation of the GTPase substrate profiles glucosylated in human colonic (Caco-2) cells treated with either TcdA, TcdB, or the related Clostridium sordellii lethal toxin (TcsL), performed by using multiple reaction monitoring (MRM) mass spectrometry. TcdA (not TcdB) glucosylates Ras subtype GTPases correlating with the fact that TcdB (not TcdA) is primarily responsible for inflammatory responses in Clostridioides difficile infection (CDI) | Clostridioides difficile |
| physiological function | quantitative evaluation of the GTPase substrate profiles glucosylated in human colonic (Caco-2) cells treated with either TcdA, TcdB, or the related Clostridium sordellii lethal toxin (TcsL), performed by using multiple reaction monitoring (MRM) mass spectrometry | Paeniclostridium sordellii |
| physiological function | quantitative evaluation of the GTPase substrate profiles glucosylated in human colonic (Caco-2) cells treated with either TcdA, TcdB, or the related Clostridium sordellii lethal toxin (TcsL), performed by using multiple reaction monitoring (MRM) mass spectrometry. TcdA (not TcdB) glucosylates Ras subtype GTPases correlating with the fact that TcdB (not TcdA) is primarily responsible for inflammatory responses in Clostridioides difficile | Clostridioides difficile |
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