Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.B62 extracted from

  • Genth, H.; Schelle, I.; Just, I.
    Metal ion activation of Clostridium sordellii lethal toxin and Clostridium difficile toxin B (2016), Toxins, 8, 109 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.B62

Protein Variants

Protein Variants Comment Organism
additional information the Dxd motif, i.e. residues D286-D288, is required at least for activation of the UDP-glucose hydrolysis activity by Mn2+ Clostridioides difficile
additional information the Dxd motif, i.e. residues D286-D288, is required at least for activation of the UDP-glucose hydrolysis activity by Mn2+ Paeniclostridium sordellii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ in addition to divalent cations, costimulation by K+ is required Clostridioides difficile
K+ in addition to divalent cations, costimulation by K+ is required Paeniclostridium sordellii
Mg2+
-
 Clostridioides difficile
Mg2+ EC50 value 0.180 mM Paeniclostridium sordellii
Mn2+
-
 Clostridioides difficile
Mn2+ EC50 value 0.028 mM Paeniclostridium sordellii
additional information divalent cation is required. Activity in decreasing order is Mn2+, Co2+, Mg2+, Ca2+, Cu2+, Zn2+. Prebound divalent metal ions are dispensible for the cytopathic effects of TcdB, it may recruit intracellular metal ions for activation of the glucosyltransferase activity Clostridioides difficile
additional information divalent cation is required. Activity in decreasing order is Mn2+, Co2+, Mg2+, Ca2+, Cu2+, Zn2+. Prebound divalent metal ions are dispensible for the cytopathic effects of TcsL, it may recruit intracellular metal ions for activation of the glucosyltransferase activity Paeniclostridium sordellii

Organism

Organism UniProt Comment Textmining
Clostridioides difficile P18177 cf. EC 3.1.4, in the absence of substrate GTPase, the enzyme exhibits UDP-hexose hydrolysis activity
-
Paeniclostridium sordellii V5T923 cf. EC 3.1.4, in the absence of substrate GTPase, the enzyme exhibits UDP-hexose hydrolysis activity
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + a small GTPase
-
 Clostridioides difficile UDP + D-glucosyl-[a small GTPase]
-
 ?
UDP-alpha-D-glucose + a small GTPase
-
 Paeniclostridium sordellii UDP + D-glucosyl-[a small GTPase]
-
 ?

Synonyms

Synonyms Comment Organism
cytotoxin B
-
 Clostridioides difficile
cytotoxin L
-
 Paeniclostridium sordellii
TcdB
-
 Clostridioides difficile
TcsL
-
 Paeniclostridium sordellii