Protein Variants | Comment | Organism |
---|---|---|
C698A | mutant is able to glucosylate Rac protein but does not display cytotoxicity. Mutant does not show autocatalytical activity | Clostridioides difficile |
D587N | mutant is able to glucosylate Rac protein but does not display cytotoxicity. Mutant does not show autocatalytical activity | Clostridioides difficile |
H653A | mutant is able to glucosylate Rac protein but does not display cytotoxicity. Mutant does not show autocatalytical activity | Clostridioides difficile |
additional information | expression of a fragment of toxin B, covering residues 1955. Wild-type fragment of toxin B is always recovered as a cleaved product with fragments of 63 and 47 kDa, whereas the mutants with changes C698A or H653A are expressed in full length | Clostridioides difficile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | P16154 | toxin A | - |
Clostridioides difficile | P18177 | toxin B | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | in presence of dithiothreitol or 2-mercaptoethanol, toxin A undergoes autocatalytic cleavage to an N-terminal catalytic domain of 60 kDa and a C-terminal part of 250 kDa. N-ethylmaleimide or iodoacetamide block this cleavage | Clostridioides difficile |
proteolytic modification | in presence of dithiothreitol or 2-mercaptoethanol, toxin B undergoes autocatalytic cleavage with release of the catalytic domain and a fragment of 210 kDa. Myo-inositol hexakisphosphate also facilitates cleavage and has a synergistic effect with dithiothreitol. Co-treatment of toxin B with N-ethylmaleimide blocks the processing induced by myo-inositol hexakisphosphate | Clostridioides difficile |
General Information | Comment | Organism |
---|---|---|
metabolism | cytotoxin A possesses an inherent cysteine protease activity, which is responsible for auto-cleavage of glucosylating toxins | Clostridioides difficile |
metabolism | cytotoxin B possesses an inherent cysteine protease activity, which is responsible for auto-cleavage of glucosylating toxins | Clostridioides difficile |