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Literature summary for 2.4.1.B53 extracted from

  • Ohashi, T.; Hasegawa, Y.; Misaki, R.; Fujiyama, K.
    Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast (2016), Appl. Microbiol. Biotechnol., 100, 687-696.
    View publication on PubMed
Search for more literature for 2.4.1.B53

Application

Application Comment Organism
synthesis naringin and narirutin are produced from naringenin-7-O-glucoside using engineered fission yeast expressing the Arabidopsis thaliana rhamnose synthase RHM2 and the Citrus maxima alpha1,2-rhamnosyltransferase or the Citrus sinensis alpha16rhamnosyl transferase genes as a whole-cell-biocatalyst Citrus sinensis
synthesis naringin and narirutin are produced from naringenin-7-O-glucoside using engineered fission yeast expressing the Arabidopsis thaliana rhamnose synthase RHM2 and the Citrus maxima alpha1,2-rhamnosyltransferase or the Citrus sinensis alpha16rhamnosyl transferase genes as a whole-cell-biocatalyst Citrus maxima

Cloned(Commentary)

Cloned (Comment) Organism
expression in Schizosaccharomyces pombe Citrus sinensis
expression in Schizosaccharomyces pombe Citrus maxima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Citrus maxima

Organism

Organism UniProt Comment Textmining
Citrus maxima A0A0P0YNP7
-
-
Citrus sinensis A7ISD3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: UDP-Xyl, UDP-Glc Citrus sinensis ?
-
 ?
additional information no substrates: UDP-Xyl, UDP-Glc Citrus maxima ?
-
 ?
UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside
-
 Citrus sinensis UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside
-
 Citrus maxima UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
UDP-L-rhamnose + quercetin 3-O-beta-D-glucoside
-
 Citrus sinensis UDP + quercetin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
UDP-L-rhamnose + quercetin 3-O-beta-D-glucoside
-
 Citrus maxima UDP + quercetin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Citrus maxima