Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.B52 extracted from

  • Simmons, T.; Mohler, K.; Holland, C.; Goubet, F.; Frankova¡, L.; Houston, D.; Hudson, A.; Meulewaeter, F.; Fry, S.
    Hetero-trans-beta-glucanase, an enzyme unique to Equisetum plants, functionalizes cellulose (2015), Plant J., 83, 753-769.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.B52

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Equisetum fluviatile

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure suggests that key amino acid substitutions Trp-Pro, Gly-Ser and Arg-Leu are responsible for the evolution of HTGÂ’s specificity from the xyloglucan-acting homo-transglycanases Equisetum fluviatile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00052
-
 (X)3-D-glucitol pH 5.5, 20°C Equisetum fluviatile
0.0014
-
 (X)3-D-Glc pH 5.5, 20°C Equisetum fluviatile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, SDS-PAGE Equisetum fluviatile

Organism

Organism UniProt Comment Textmining
Equisetum fluviatile A0A0K2WA62 bifunctional xyloglucan endotransglucosylase, EC 2.4.1.207, and mixed-linkage glucan:xyloglucan endotransglucosylase, ratio of the activities is 1:7, respectively
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Equisetum fluviatile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mixed-linkage (1->3, 1->4)-beta-D-glucan + XXXGol
-
 Equisetum fluviatile ?
-
 ?
additional information enzyme predominantly catalyzes hetero-transglycosylation. Its preferred donor substrates (cellulose or mixed-linkage beta-glucan) differ qualitatively from its acceptor substrate xyloglucan. HTG generates stable cellulose-xyloglucan and mixed-linkage beta-glucan-xyloglucan covalent bonds. No donor substrate: cellohexaose Equisetum fluviatile ?
-
 ?
tamarind xyloglucan + XXXG
-
 Equisetum fluviatile ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Equisetum fluviatile

Synonyms

Synonyms Comment Organism
HTG
-
 Equisetum fluviatile

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0044
-
 (X)3-D-glucitol pH 5.5, 20°C Equisetum fluviatile

pI Value

Organism Comment pI Value Maximum pI Value
Equisetum fluviatile isoelectric focusing
-
 4.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.5
-
 (X)3-D-glucitol pH 5.5, 20°C Equisetum fluviatile