Literature summary for 2.4.1.B34 extracted from

Bai, Y.; Gangoiti, J.; Dijkstra, B.W.; Dijkhuizen, L.; Pijning, T.
Crystal structure of 4,6-alpha-glucanotransferase supports diet-driven evolution of GH70 enzymes from alpha-amylases in oral bacteria (2017), Structure, 25, 231-242 .

Search for more literature for 2.4.1.B34

Cloned(Commentary)

Cloned (Comment)	Organism
gene gtfB, genomic mapping and phylogenetic analysis, recombinant expression of wild-type and truncated mutant enzymes	Limosilactobacillus reuteri

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type enzyme in apoform and in complex with maltohexaose, and enzyme mutant D1015N in complex with maltopentaose, crystallized by the hanging-drop vapor diffusion method and a microseeding protocol, X-ray diffraction structre determination and analysis at 1.80-2.19 A resolution, molecular replacement using domains A, B, C, and IV of the crystal structure of Lactobacillus reuteri 180 GTF180-DELTAN (PDB ID 3KLK) as a search model	Limosilactobacillus reuteri

Crystallization (Comment)

Organism

purified recombinant wild-type enzyme in apoform and in complex with maltohexaose, and enzyme mutant D1015N in complex with maltopentaose, crystallized by the hanging-drop vapor diffusion method and a microseeding protocol, X-ray diffraction structre determination and analysis at 1.80-2.19 A resolution, molecular replacement using domains A, B, C, and IV of the crystal structure of Lactobacillus reuteri 180 GTF180-DELTAN (PDB ID 3KLK) as a search model

Limosilactobacillus reuteri

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of deletion GtfB-DELTANDELTAV mutant D1015N	Limosilactobacillus reuteri

Organism

Organism	UniProt	Comment	Textmining
Limosilactobacillus reuteri	A0A0U5F702	-	-
Limosilactobacillus reuteri 121	A0A0U5F702	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	malto-oligosaccharide binding structures with wild-type and mutant enzymes, overview	Limosilactobacillus reuteri	?	-	-
additional information	malto-oligosaccharide binding structures with wild-type and mutant enzymes, overview	Limosilactobacillus reuteri 121	?	-	-

Synonyms

Synonyms	Comment	Organism
GtfB	-	Limosilactobacillus reuteri
UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase stabilizing protein	UniProt	Limosilactobacillus reuteri

General Information

General Information	Comment	Organism
evolution	the crystal structure analysis of 4,6-alpha-glucanotransferase supports diet-driven evolution of GH70 enzymes from alpha-amylases in oral bacteria, overview. Mode of action and detailing the structural changes accompanying the proposed evolution of glycoside hydrolase family 70 (GH70). The enzyme belongs to the glycoside hydrolase family 70 (GH70)	Limosilactobacillus reuteri
additional information	structure-function analysis, modeling and docking, overview. Mechanism and mode of action of GtfB in comparison with alpha-amylase and glucansucrase	Limosilactobacillus reuteri