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Literature summary for 2.4.1.B34 extracted from
- Characterization of the Paenibacillus beijingensis DSM 24997 GtfD and its glucan polymer products representing a new glycoside hydrolase 70 subfamily of 4,6-alpha-glucanotransferase enzymes (2017), PLoS ONE, 12, e0172622 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gtfD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His6-tagged full-length GtfD enzyme without its putative signal peptide-encoding sequence (amino acids 31 to 776) in Escherichia coli strain BL21 Star (DE3) | Paenibacillus beijingensis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Paenibacillus beijingensis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus beijingensis | - |
- |
- |
| Paenibacillus beijingensis DSM 24997 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged GtfD enzyme without its putative signal peptide-encoding sequence (amino acids 31 to 776) from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography and ultrafiltration | Paenibacillus beijingensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
purified recombinant enzyme, pH 7.0, 50°C, 1 mM CaCl2 | Paenibacillus beijingensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | hydrolytic activity | Paenibacillus beijingensis | ? | - |
? | |
| amylose V + H2O | hydrolytic activity | Paenibacillus beijingensis | ? | - |
? | |
| amylose V + maltoheptaose | transglycosylation activity | Paenibacillus beijingensis | ? | - |
? | |
| amylose V + maltohexaose | transglycosylation activity | Paenibacillus beijingensis | ? | - |
? | |
| amylose V + maltopentaose | transglycosylation activity | Paenibacillus beijingensis | ? | - |
? | |
| amylose V + maltotetraose | transglycosylation activity | Paenibacillus beijingensis | ? | - |
? | |
| amylose V + maltotriose | transglycosylation activity | Paenibacillus beijingensis | ? | - |
? | |
| maltoheptaose + H2O | hydrolytic activity | Paenibacillus beijingensis | ? | - |
? | |
| maltoheptaose + H2O | hydrolytic activity | Paenibacillus beijingensis DSM 24997 | ? | - |
? | |
| maltohexaose + H2O | hydrolytic activity | Paenibacillus beijingensis | ? | - |
? | |
| maltohexaose + H2O | hydrolytic activity | Paenibacillus beijingensis DSM 24997 | ? | - |
? | |
| maltopentaose + H2O | hydrolytic activity | Paenibacillus beijingensis | ? | - |
? | |
| maltopentaose + H2O | hydrolytic activity | Paenibacillus beijingensis DSM 24997 | ? | - |
? | |
| maltotetraose + H2O | hydrolytic activity | Paenibacillus beijingensis | ? | - |
? | |
| maltotetraose + H2O | hydrolytic activity | Paenibacillus beijingensis DSM 24997 | ? | - |
? | |
| maltotriose + H2O | hydrolytic activity | Paenibacillus beijingensis | ? | - |
? | |
| additional information | the enzyme GtfD shows both hydrolysis and transglycosylase (disproportionation) activity. 1HNMR analysis of the product mixture generated from amylose V reveals the presence of two broad anomeric signals corresponding to the (alpha1->4) and the newly formed (alpha1->6) linkages. Substrate specificity with maltooligosaccharides, overview. The Paenibacillus beijingensis GtfD enzyme is inactive on sucrose, panose, nigerose, and isomalto-oligosaccharides with DP2, DP3, and DP5, while the enzyme catalyzes the conversion of malto-oligosaccharides (MOS) of DP3 to 7 showing both hydrolysis and transglycosylase (disproportionation) activity revealing the formation of lower- and higher-molecular-mass products | Paenibacillus beijingensis | ? | - |
- |
|
| additional information | the enzyme GtfD shows both hydrolysis and transglycosylase (disproportionation) activity. 1HNMR analysis of the product mixture generated from amylose V reveals the presence of two broad anomeric signals corresponding to the (alpha1->4) and the newly formed (alpha1->6) linkages. Substrate specificity with maltooligosaccharides, overview. The Paenibacillus beijingensis GtfD enzyme is inactive on sucrose, panose, nigerose, and isomalto-oligosaccharides with DP2, DP3, and DP5, while the enzyme catalyzes the conversion of malto-oligosaccharides (MOS) of DP3 to 7 showing both hydrolysis and transglycosylase (disproportionation) activity revealing the formation of lower- and higher-molecular-mass products | Paenibacillus beijingensis DSM 24997 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GtfD | - |
Paenibacillus beijingensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Paenibacillus beijingensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Paenibacillus beijingensis |
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Release 2023.1 (January 2023)
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